Id |
Subject |
Object |
Predicate |
Lexical cue |
T206 |
0-70 |
Sentence |
denotes |
Figure 5 Colocalization of CK2 and Viral Proteins at Actin Protrusions |
T207 |
71-176 |
Sentence |
denotes |
(A) Pathway of regulated PHs and SARS-CoV-2 interaction partners involved in cytoskeletal reorganization. |
T208 |
177-279 |
Sentence |
denotes |
Dashed lines indicate downregulation of activity, while solid lines indicate upregulation of activity. |
T209 |
280-348 |
Sentence |
denotes |
(B) Regulation of individual kinase activity or PHs depicted in (A). |
T210 |
349-481 |
Sentence |
denotes |
(C) Caco-2 cells infected with SARS-CoV-2 at an MOI of 0.1 for 24 h prior to immunostaining for F-actin and M protein, as indicated. |
T211 |
482-628 |
Sentence |
denotes |
Shown is a confocal section revealing M protein localization along and to the tip of filopodia (left) and magnification of the dashed box (right). |
T212 |
629-765 |
Sentence |
denotes |
(D) Dot plot quantification of the number and length of filopodia in untreated (mock) or infected Caco-2 cells for 24 h with SARS-CoV-2. |
T213 |
766-850 |
Sentence |
denotes |
Filopodium length was measured from the cortical actin to the tip of the filopodium. |
T214 |
851-875 |
Sentence |
denotes |
Error bars represent SD. |
T215 |
876-917 |
Sentence |
denotes |
Statistical testing by Mann-Whitney test. |
T216 |
918-1082 |
Sentence |
denotes |
(E) Caco-2 cells infected with SARS-CoV-2 at an MOI of 0.01 for 24 h prior to immunostaining for F-actin, N protein, and casein kinase II (CK2) as indicated (left). |
T217 |
1083-1151 |
Sentence |
denotes |
Shown is magnification of the dashed box as single channels (right). |
T218 |
1152-1294 |
Sentence |
denotes |
(F) Magnification of the dashed box from (E) with quantification of colocalization between CK2 and N protein throughout infected Caco-2 cells. |
T219 |
1295-1377 |
Sentence |
denotes |
Displayed is the proportion of N protein-positive particles colocalizing with CK2. |
T220 |
1378-1402 |
Sentence |
denotes |
Error bars represent SD. |
T221 |
1403-1563 |
Sentence |
denotes |
(G and H) Scanning electron microscopy (G) and transmission electron microscopy (H) images of SARS-CoV-2 budding from Vero E6 cell filopodia (black arrow in H). |
T222 |
1564-1583 |
Sentence |
denotes |
See also Figure S3. |