| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T120 |
0-136 |
Sentence |
denotes |
Finally, phosphorylation sites in N protein, a structural protein that binds to and assists with packaging viral RNA, were investigated. |
| T121 |
137-285 |
Sentence |
denotes |
Most sites occurred within the N-terminal portion of the protein, at or near the RNA binding region, but avoided the C-terminal dimerization domain. |
| T122 |
286-472 |
Sentence |
denotes |
The cluster of phosphorylation sites within an arginine/serine (RS)-dipeptide rich region, C-terminal to the RNA binding region (Figure 2A), is conserved in other coronavirus N proteins. |
| T123 |
473-649 |
Sentence |
denotes |
This region is phosphorylated in SARS-CoV by serine-arginine (SR) protein kinases, modulating the role of SARS-CoV N protein in host translation inhibition (Peng et al., 2008). |
| T124 |
650-739 |
Sentence |
denotes |
It is likely that phosphorylation of this same region in SARS-CoV-2 plays a similar role. |
| T125 |
740-953 |
Sentence |
denotes |
Interestingly, in vitro inhibition of SARS-CoV N protein phosphorylation at the RS-rich region results in reduced viral load and cytopathic effects (Wu et al., 2009), highlighting its importance for viral fitness. |
| T126 |
954-1096 |
Sentence |
denotes |
In addition, sites spanning the sequence of the RNA binding domain, which forms a claw-like structure, have been observed (Kang et al., 2020). |
| T127 |
1097-1310 |
Sentence |
denotes |
Several phosphorylation sites cluster in the structural model, predicted to affect the surface charge of the so-called acidic wrist region (Figure 2F) but not the positive surface charge of the RNA binding pocket. |
| T128 |
1311-1457 |
Sentence |
denotes |
We hypothesize that this surface charge difference may modulate N protein function, potentially via allosteric regulation of RNA binding capacity. |
