LitCovid-sentences  

PMC:7311890 / 55616-57803 JSONTXT 10 Projects

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Id Subject Object Predicate Lexical cue
T417 0-76 Sentence denotes All blood samples used in this study were collected after an overnight fast.
T418 77-167 Sentence denotes Peripheral blood samples from patients were collected within 24 h upon hospital admission.
T419 168-287 Sentence denotes For patients admitted in the evening, blood samples were taken on the next morning (0500-0600) before breakfast (0700).
T420 288-456 Sentence denotes For patients admitted during daytime, blood samples were collected only on the next morning (0500-0600) before breakfast (0700) (i.e., after one night in the hospital).
T421 457-588 Sentence denotes Throughout the hospitalization period, patients were provided two standard meals per day, scheduled at 0700 and 1700, respectively.
T422 589-638 Sentence denotes Blood was collected in BD Vacutainer (BD 367525).
T423 639-701 Sentence denotes Plasma was separated by centrifugation at 2000 rpm for 10 min.
T424 702-823 Sentence denotes Lipids and metabolites were extracted according to a modified version of the Bligh and Dyer’s protocol (Lu et al., 2019).
T425 824-969 Sentence denotes Plasma (100 μL) for metabolomics and lipidomics analyses was inactivated via the addition of 750 μL of ice-cold chloroform: methanol (1:2) (v/v).
T426 970-1047 Sentence denotes Samples were vortexed for 15 s and then incubated for 1 h at 1500 rpm at 4°C.
T427 1048-1151 Sentence denotes At the end of incubation, 250 μL of ice-cold chloroform and 350 μL of ice-cold MilliQ water were added.
T428 1152-1208 Sentence denotes Samples were vortexed for 15 s and put on ice for 1 min.
T429 1209-1237 Sentence denotes This step was repeated once.
T430 1238-1323 Sentence denotes Samples were then centrifuged at 12 000 rpm for 5 min 4°C to induce phase separation.
T431 1324-1382 Sentence denotes The lower organic phase was first extracted to a new tube.
T432 1383-1477 Sentence denotes Then, another 450 μL of ice-cold chloroform was added to the remaining aqueous/methanol phase.
T433 1478-1587 Sentence denotes Samples were vortexed briefly for 15 s and put on ice for 1 min, and centrifuged at 12 000 rpm for 5 min 4°C.
T434 1588-1683 Sentence denotes The lower organic phase was extracted and pooled together with the first round organic extract.
T435 1684-1775 Sentence denotes Double rounds of extraction ensured a better recovery and reduce variations across samples.
T436 1776-1957 Sentence denotes The remaining aqueous/methanol phase was then centrifuged at 12 000 rpm for 5 min 4°C, and clean supernatant containing polar metabolites were extracted and transferred to new tube.
T437 1958-2062 Sentence denotes The organic phase was dried in the SpeedVac under OH mode, while aqueous phase was dried under H2O mode.
T438 2063-2187 Sentence denotes The dried metabolite extracts were shipped on dry ice to the designated laboratory for lipidomics and metabolomics analysis.