LitCovid-sentences  

PMC:7311890 / 36123-39786 JSONTXT 10 Projects

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Id Subject Object Predicate Lexical cue
T227 0-95 Sentence denotes Lipid analyses were conducted on exosomes isolated from the plasma of the same cohort (n = 75).
T228 96-264 Sentence denotes The purity of isolated exosomes was validated by the enrichment in exosome-specific protein marker ALG-2-interacting protein X (Alix) (Théry et al., 2001) (Figure S5A).
T229 265-481 Sentence denotes In line with previous reports on exosome lipid compositions (Subra et al., 2007), the isolated exosomes displayed selective enrichment of raft-associated lipids, including free cholesterol (Cho) and SMs (Figure S5B).
T230 482-687 Sentence denotes Interestingly, we also noted exosome-specific enrichments in 20:4-BMPs, PUFA-PSs, and several GM3s relative to plasma (Figure S5B), suggesting that these lipids might partake in exosome-specific processes.
T231 688-893 Sentence denotes In corroboration with postulations drawn based on plasma lipid profile changes, we found that GM3s were increasingly enriched in the exosomes of COVID-19 patients of elevating disease severity (Figure 5 ).
T232 894-1036 Sentence denotes Several GM3s exhibited greater than 2-fold increases in the exosomes (p < 0.05) of COVID-19 patients relative to healthy controls (Figure 5A).
T233 1037-1308 Sentence denotes Progressive increases with increasing disease severity were similarly noted for GM3s of varying acyl chain lengths, e.g., very long-chain GM3 d18:0/26:0 (p = 0.0008) and GM3 d18:1/24:1 (p = 0.0015), as well as medium-chain such as GM3 d18:1/16:0 (p = 0.0017) (Figure 5B).
T234 1309-1410 Sentence denotes BMP38:5(18:1/20:4) was reduced in the exosomes of COVID-19 patients compared to controls (Figure 5A).
T235 1411-1548 Sentence denotes Increasing endosomal BMP content was previously found to impede intercellular transmission of HIV particles (Chapuy-Regaud et al., 2013).
T236 1549-1679 Sentence denotes PUFA-PSs such as PS 40:7 and PS 40:5 were specifically increased in the exosomes of severe compared to moderate cases (Figure 5C).
T237 1680-1874 Sentence denotes While plasma changes in sphingolipids, particularly GM3s, were recapitulated in exosomes, many of the changes in phospholipids (with the exception of PSs) were not observed in isolated exosomes.
T238 1875-2055 Sentence denotes These observations suggested that plasma changes in glycerophospholipids associated with COVID-19 might be attributed to other components, such as lipoproteins, in the circulation.
T239 2056-2111 Sentence denotes Figure 5 Lipid Changes in Exosomes of COVID-19 Patients
T240 2112-2223 Sentence denotes (A) Lipid changes in isolated exosomes from plasma of healthy controls (n = 25) and COVID-19 patients (n = 50).
T241 2224-2376 Sentence denotes Colored dots (blue and red) in volcano plot indicate lipids that were significantly different (p < 0.05) between healthy controls and COVID-19 patients.
T242 2377-2634 Sentence denotes Blue dots indicate significant lipids with fold change ≥ 2 in COVID-19 patients relative to controls listed in the blue panel, while red dots indicate significant lipids with fold change < 2 in patients relative to controls (listed in red and green panels).
T243 2635-2865 Sentence denotes Lipids on the right side of vertical line at x = 0 were increased in COVID-19 patients compared to controls (blue and red panels), and lipids on the left side were decreased in COVID-19 patients compared to controls (green panel).
T244 2866-3158 Sentence denotes (B) Boxplots illustrate sum of all GM3s (p = 0.0037), and three representatives, GM3 d18:0/26:0 (p = 0.0008), GM3 d18:1/16:0 (p = 0.0017), and GM3 d18:1/24:1 (p = 0.0015), that displayed increasing trends in isolated exosomes from healthy controls to COVID-19 patients of increasing severity.
T245 3159-3255 Sentence denotes Exosome lipids were expressed in nanomoles of lipids per gram of total protein (nmol/g protein).
T246 3256-3399 Sentence denotes (C) Volcano plots illustrate exosome lipids that were significantly different (p < 0.05) in pairwise comparisons indicated on top of each plot.
T247 3400-3643 Sentence denotes Dots corresponding to significant lipids (p < 0.05) were colored (blue and red), and lipids with fold change ≥ 2 were colored blue and listed in the blue panel accompanying each volcano plot, while lipids with fold change < 2 were colored red.
T248 3644-3663 Sentence denotes See also Figure S5.