LitCovid-sentences  

PMC:7299399 / 49393-53465 JSONTXT 11 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T191 0-41 Sentence denotes Nanotechnology Tools to Detect SARS-CoV-2
T192 42-372 Sentence denotes Standard procedures for detecting the virus from nasopharyngeal and/or oropharyngeal swabs have been reviewed recently and are primarily based on reverse transcription polymerase chain reaction (RT-PCR).140 Here, we would like to mention some preliminary ideas on nanotechnology-based assays to monitor the presence of SARS-CoV-2.
T193 373-568 Sentence denotes A simplified test and variants thereof to detect viral proteins (e.g., HIV or influenza virus) without the need for expensive equipment is based on the color change of Au NPs bound to antibodies.
T194 569-975 Sentence denotes Similar to the enzyme-linked immunosorbent assay (ELISA) antibodies coupled to Au NPs will form a tertiary complex with the viral antigen and a capture antibody, thereby leading to the immobilization and agglomeration of NPs, which shifts the color from red to blue.32 Such simple, low-cost procedures could be of value in regions with low-resource medical infrastructure, as found in developing countries.
T195 976-1404 Sentence denotes For rapid, on-site detection, the development of graphene-based field-effect transistor (FET) biosensing devices coupled to a specific antibody against SARS-CoV-2 spike protein was recently reported.141 Dual-functional plasmonic biosensors combining the plasmonic photothermal (PPT) effect and localized surface plasmon resonance were also recently developed and could be used as a cost-effective and fast alternative to RT-PCR.
T196 1405-1519 Sentence denotes Here, Au nanomaterials coupled to complementary DNA sequences are used to sense hybridized cDNAs of SARS-CoV-2.142
T197 1520-1840 Sentence denotes Nanobiosensors are a valuable alternative to conventional laboratory equipments for clinical and environmental analyses.143 Usually, nanobiosensors combine the excellent electrical and optical properties of nanomaterials with biological or synthetic molecules used as receptors to detect selectively any kind of analyte.
T198 1841-2104 Sentence denotes The detection of whole cells (e.g., cancer cells) using electrocatalytic properties of Au NPs toward hydrogen evolution has been reported.144 This cell-sensing device is based on the reaction of cell surface proteins with specific antibodies conjugated to Au NPs.
T199 2105-2248 Sentence denotes The same detection technique can also be applied to the detection of viruses, taking advantages of the known antigens and available antibodies.
T200 2249-2464 Sentence denotes Recently, as mentioned above, a FET sensor (modified with graphene sheets) that uses a specific antibody against SARS-CoV-2 spike protein was reported to detect SARS-CoV-2 in culture medium (limit of detection, LOD:
T201 2465-2509 Sentence denotes 1.6 × 101 pfu/mL) and clinical samples (LOD:
T202 2510-2535 Sentence denotes 2.42 × 102 copies/mL).141
T203 2536-2716 Sentence denotes Researchers have also demonstrated solid-phase isothermal recombinase polymerase amplification (RPA) for the detection of Citrus tristeza virus (CTV) for plant disease diagnostics.
T204 2717-3038 Sentence denotes Gold NPs linked with DNA strands on top of an electrode were used to detect in situ amplified CTV (RPA assay for amplification of the P20 gene, 387 bp) using electrochemical impedance spectroscopy (EIS) in a Fe(CN6)4–/Fe(CN6)3– redox system, being able to detect 1000 fg μL–1 of nucleic acid quantitatively (Figure 4).145
T205 3039-3236 Sentence denotes Figure 4 Schematic of recombinase polymerase amplification-based Citrus tristeza virus detection using gold-nanoparticle-modified DNA strands and electrochemical impedance spectroscopic detection.
T206 3237-3262 Sentence denotes Reprinted from ref (145).
T207 3263-3304 Sentence denotes Copyright 2019 American Chemical Society.
T208 3305-3697 Sentence denotes Paper/nanomaterial-based sensors,146,147 in general, and, particularly, NP-based lateral-flow devices that are observed with the naked eye or operated through a smartphone are now the most reported testing platforms for the analysis of COVID-19-related biomarkers.148 Nanopaper147 and nanochannels149 may also offer cost-efficient alternatives for detection of viruses and related biomarkers.
T209 3698-3764 Sentence denotes Nanomaterials are already being used in theranostics applications.
T210 3765-4072 Sentence denotes Although the field of nanomaterial applications in nanotheranostics is still in its infancy,55 a thoughtful review of the performance characteristics of known nanomaterials may open new avenues of investigation, even for COVID-19-related applications (for both diagnostic and therapies, including vaccines).