| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T574 |
0-214 |
Sentence |
denotes |
The thermal stabilization of the native protein structure upon ligand binding was monitored by differential scanning fluorimetry, performed according to the method described previously [34] with some modifications. |
| T575 |
215-432 |
Sentence |
denotes |
Reaction mixture (25 μL) consisted of 20 mM HEPES (pH 7.5), 150 mM NaCl, 1X SYPRO orange dye (Invitrogen), 5 μM SARS-CoV nsp14 protein and 1 mM compound previously dissolved in 100% DMSO or water (SAM and Sinefungin). |
| T576 |
433-559 |
Sentence |
denotes |
The protein was first mixed with compound before the addition of the dye and incubated in 96-well white microplates (Bio-Rad). |
| T577 |
560-696 |
Sentence |
denotes |
Fluorescence intensities were measured from 20 to 95 °C with a ramp rate of 0.2 °C/min using a Real-Time PCR detection system (Bio-Rad). |
| T578 |
697-757 |
Sentence |
denotes |
Control reactions were performed in the presence of 5% DMSO. |
| T579 |
758-919 |
Sentence |
denotes |
Fluorescence intensities were plotted as a function of temperature and melting temperature (T m) was determined by curve-fitting using Prism (GraphPad software). |
| T580 |
920-1103 |
Sentence |
denotes |
The apparent affinity constant (KD) for compound 13 was calculated by nonlinear regression analysis with one site-specific binding equation Hill slope using Prism (GraphPad software). |
