PMC:7291971 / 85080-85465 JSONTXT 10 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T559 0-155 Sentence denotes SARS-CoV nsp14 and human RNA N7-methyltransferase (hRNMT) coding sequences were cloned in fusion with a N-terminus hexa-histidine tag in Gateway® plasmids.
T560 156-257 Sentence denotes The proteins were expressed in E. coli and purified following previously described protocols [33,39].
T561 258-385 Sentence denotes Vaccinia virus capping enzyme (D1/D12 complex) and mRNA Cap 2′-O-methyltransferase (VP39) were purchased (New England Biolabs).