Id |
Subject |
Object |
Predicate |
Lexical cue |
T559 |
0-155 |
Sentence |
denotes |
SARS-CoV nsp14 and human RNA N7-methyltransferase (hRNMT) coding sequences were cloned in fusion with a N-terminus hexa-histidine tag in Gateway® plasmids. |
T560 |
156-257 |
Sentence |
denotes |
The proteins were expressed in E. coli and purified following previously described protocols [33,39]. |
T561 |
258-385 |
Sentence |
denotes |
Vaccinia virus capping enzyme (D1/D12 complex) and mRNA Cap 2′-O-methyltransferase (VP39) were purchased (New England Biolabs). |