| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T558 |
0-56 |
Sentence |
denotes |
4.2 Expression and purification of recombinant proteins |
| T559 |
57-212 |
Sentence |
denotes |
SARS-CoV nsp14 and human RNA N7-methyltransferase (hRNMT) coding sequences were cloned in fusion with a N-terminus hexa-histidine tag in Gateway® plasmids. |
| T560 |
213-314 |
Sentence |
denotes |
The proteins were expressed in E. coli and purified following previously described protocols [33,39]. |
| T561 |
315-442 |
Sentence |
denotes |
Vaccinia virus capping enzyme (D1/D12 complex) and mRNA Cap 2′-O-methyltransferase (VP39) were purchased (New England Biolabs). |
