| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T53 |
0-170 |
Sentence |
denotes |
We thus synthesized a new series of transition state analogs of the RNA 2′-O-methylation reaction based on the coupling of a 2′-O-ethyl adenosine to a 5′-amino adenosine. |
| T54 |
171-366 |
Sentence |
denotes |
We explored a variety of N-substituted linkers in adenine dinucleosides and their inhibition activity was evaluated against several viral RNA 2′O-MTases as well N7-MTases for specificity purpose. |
| T55 |
367-469 |
Sentence |
denotes |
Unexpectedly, none of the N-linked dinucleosides inhibited any 2′O-MTases of flaviviruses or SARS-CoV. |
| T56 |
470-675 |
Sentence |
denotes |
However, interestingly some N-nitrobenzenesulfonamide-containing dinucleosides were found to specifically inhibit SARS-CoV N7-MTase nsp14 without inhibiting the cognate human N7-MTase or vaccinia N7-MTase. |
| T57 |
676-786 |
Sentence |
denotes |
Such specific inhibition results from a high binding affinity of the most potent inhibitors to N7-MTase nsp14. |
| T58 |
787-915 |
Sentence |
denotes |
In addition, computational docking analysis identified some residues of the binding site for the best inhibitor targeting nsp14. |
| T59 |
916-1282 |
Sentence |
denotes |
As SAM and RNA binding sites of N7-MTase nsp14 are almost completely conserved between SARS-CoV and SARS-CoV-2 (95% sequence homology, Supporting Info Fig. S1) [18], we can forecast that the candidate ligands that are efficient in inhibiting the SARS-CoV functions will be efficient in doing the same for SARS-CoV-2, this emphasizes the interest of the present work. |
