| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T646 |
0-146 |
Sentence |
denotes |
Most of the studies have used real-time RT-PCR with matrix-gene specific oligonucleotide primers and TaqMan probe for IAV detection [308,341,342]. |
| T647 |
147-261 |
Sentence |
denotes |
The conserved sequences of the matrix-gene specific primers can detect any IAV subtype in the swine samples [343]. |
| T648 |
262-584 |
Sentence |
denotes |
Most of the studies used subtype-specific real-time RT-PCR for the IAV subtyping, however, few studies opted for the conventional approach of reverse-transcription PCR followed by Sanger sequencing for amplification of the HA and/or NA genes for retrieving the sequences for phylogenetic analyses to identify the subtypes. |
| T649 |
585-730 |
Sentence |
denotes |
Although the real-time RT-PCR is a powerful and rapid tool for the subtyping of IAV strains, it is more expensive than reverse-transcription PCR. |
| T650 |
731-1025 |
Sentence |
denotes |
A few studies have reported reverse-transcription PCR based amplification of all the eight gene segments of IAV to generate the whole genome sequences [33,91,128,231] but in most cases, the whole genome sequences were generated using MiSeq next-generation sequencing approach [150,214,325,329]. |
| T651 |
1026-1153 |
Sentence |
denotes |
A great advantage of this sequencing approach is that it can identify novel influenza viruses in the swine samples [80,90,301]. |
