Id |
Subject |
Object |
Predicate |
Lexical cue |
T135 |
0-20 |
Sentence |
denotes |
Epitope predictions: |
T136 |
21-194 |
Sentence |
denotes |
Thirty one linear B-cell epitopes were predicted by Bepipred in the Indian SARS-CoV-2, of which three were found to have a length of <6 amino acids and hence not considered. |
T137 |
195-324 |
Sentence |
denotes |
Linear epitopes were also predicted using the ABCpred prediction tool, which predicted 47 epitopes based on the threshold of 0.8. |
T138 |
325-403 |
Sentence |
denotes |
Regions common to both the prediction methods (n=17) were identified manually. |
T139 |
404-589 |
Sentence |
denotes |
The 17 epitopes were screened for their antigenicity using the VaxiJen v2.0 tool (http://www.ddg-pharmfac.net/vaxijen/VaxiJen/VaxiJen.html), and nine of these epitopes were shortlisted. |
T140 |
590-720 |
Sentence |
denotes |
These epitopes were further screened for their ability to elicit an IFN-γ response, which was predicted using the IFNepitope tool. |
T141 |
721-901 |
Sentence |
denotes |
Finally, five epitopes, four in the S1 domain and one in the S2 domain, were predicted, which could possibly generate an immune response and suppress the IFN-γ response (Table II). |
T142 |
902-1074 |
Sentence |
denotes |
N-linked glycosylation site prediction revealed that two putative glycosylation sites (with a low value for jury agreement) were present within the epitope stretch 328-344. |