| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T160 |
0-29 |
Sentence |
denotes |
Glycan analysis by HILIC-UPLC |
| T161 |
30-242 |
Sentence |
denotes |
Labelled glycans were analysed using a 2.1 mm × 150 mm Acquity BEH Glycan column (Waters) on an Acquity H-Class UPLC instrument (Waters), with fluorescence measurements occurring at λex = 310 nm and λem = 370 nm. |
| T162 |
243-289 |
Sentence |
denotes |
The following gradient was used: time (t) = 0: |
| T163 |
290-338 |
Sentence |
denotes |
22% A, 78% B (flow rate = 0.5 mL/min); t = 38.5: |
| T164 |
339-379 |
Sentence |
denotes |
44.1% A, 55.9% B (0.5 mL/min); t = 39.5: |
| T165 |
380-417 |
Sentence |
denotes |
100% A, 0% B (0.25 mL/min); t = 44.5: |
| T166 |
418-455 |
Sentence |
denotes |
100% A, 0% B (0.25 mL/min); t = 46.5: |
| T167 |
456-558 |
Sentence |
denotes |
22% A, 78% B (0.5 mL/min), where solvent A was 50 mM ammonium formate (pH 4.4) and B was acetonitrile. |
| T168 |
559-739 |
Sentence |
denotes |
Quantification of oligomannose-type glycans was achieved by digestion of fluorescently labelled glycans with Endo H, and clean-up using a PVDF protein-binding membrane (Millipore). |
| T169 |
740-797 |
Sentence |
denotes |
Empower 3 software (Waters) was used for data processing. |
