| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T704 |
0-94 |
Sentence |
denotes |
Drop-seq experiments were performed according to the original protocol (Macosko et al., 2015). |
| T705 |
95-221 |
Sentence |
denotes |
Briefly, single cells (100/μl) were co-encapsulated in droplets with barcoded beads (120/μl, ChemGenes) at rates of 4000 μl/h. |
| T706 |
222-336 |
Sentence |
denotes |
Droplet emulsions were collected for 10-20 min/each prior to droplet breakage by perfluorooctanol (Sigma-Aldrich). |
| T707 |
337-457 |
Sentence |
denotes |
After breakage, beads were harvested and the hybridized mRNA transcripts reverse transcribed (Maxima RT, Thermo Fisher). |
| T708 |
458-534 |
Sentence |
denotes |
Exonuclease digestion and PCR were carried out as described (12 PCR cycles). |
| T709 |
535-701 |
Sentence |
denotes |
For each sample, 1 ng of pre-amplified cDNA from an estimated 1000 cells was tagmented by Nextera XT (Illumina) with a custom P5-primer (Integrated DNA Technologies). |
| T710 |
702-792 |
Sentence |
denotes |
Single-cell libraries were sequenced in a 100 bp paired-end run on the Illumina HiSeq4000. |
