| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T865 |
0-122 |
Sentence |
denotes |
Finally, we investigated how observed differences in ACE2+ proportions across clusters might be affected by cell sampling. |
| T866 |
123-545 |
Sentence |
denotes |
Using the proportion of ACE2+ cells in a “typical” cluster annotated as being ACE2 positive (i.e., 6.8% in non-human primate type II pneumocytes, Figure 1), we calculated the cluster sizes needed to be confident that the probability of observing zero to a few positive cells is unlikely to have arisen by random chance (probabilities calculated under a negative binomial distribution with parameter p = 0.068, Figure S6E). |
| T867 |
546-731 |
Sentence |
denotes |
We found that as cluster sizes approach and exceed 100 cells, the probability of observing zero to a few positive cells rapidly approaches zero, if we assume 6.8% of cells are positive. |
| T868 |
732-1349 |
Sentence |
denotes |
Further, to examine our confidence in estimating an approximate upper bound (ignoring the impact of protocol inefficiencies discussed above) for the fraction of cells positive in a cluster as a function of the number of cells in that cluster, we also calculated the probability of observing zero (and its complement, probability of observing at least 1) ACE2+ cells as a function of cluster size across true positive proportions ranging from 0.1% to 10% (probabilities calculated under a negative binomial distribution with parameter p = 0.001 to 0.1, representing hypothetical proportions of ACE2+ cells Figure S6F). |
| T869 |
1350-1590 |
Sentence |
denotes |
Given our typical cluster sizes (on the order of hundreds of cells, exact values provided in Table S9), we find that for us to observe 0 ACE2+ cells in a cluster due to sampling artifacts, the fraction of true positives must be ∼1% or less. |
| T870 |
1591-1787 |
Sentence |
denotes |
Thus, these complementary approaches demonstrate that our observed variations in ACE2+ cell proportions across clusters likely reflect underlying biological differences, rather than random chance. |
