| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-97 |
Sentence |
denotes |
Deducing the N- and O- glycosylation profile of the spike protein of novel coronavirus SARS-CoV-2 |
| T2 |
99-107 |
Sentence |
denotes |
Abstract |
| T3 |
108-116 |
Sentence |
denotes |
Abstract |
| T4 |
117-289 |
Sentence |
denotes |
The current emergence of the novel coronavirus pandemic caused by SARS-CoV-2 demands the development of new therapeutic strategies to prevent rapid progress of mortalities. |
| T5 |
290-484 |
Sentence |
denotes |
The coronavirus spike (S) protein, which facilitates viral attachment, entry and membrane fusion is heavily glycosylated and plays a critical role in the elicitation of the host immune response. |
| T6 |
485-611 |
Sentence |
denotes |
The spike protein is comprised of two protein subunits (S1 and S2), which together possess 22 potential N-glycosylation sites. |
| T7 |
612-759 |
Sentence |
denotes |
Herein, we report the glycosylation mapping on spike protein subunits S1 and S2 expressed on human cells through high resolution mass spectrometry. |
| T8 |
760-974 |
Sentence |
denotes |
We have characterized the quantitative N-glycosylation profile on spike protein and interestingly, observed unexpected O-glycosylation modifications on the receptor binding domain (RBD) of spike protein subunit S1. |
| T9 |
975-1198 |
Sentence |
denotes |
Even though O-glycosylation has been predicted on the spike protein of SARS-CoV-2, this is the first report of experimental data for both the site of O-glycosylation and identity of the O-glycans attached on the subunit S1. |
| T10 |
1199-1425 |
Sentence |
denotes |
Our data on the N- and O- glycosylation is strengthened by extensive manual interpretation of each glycopeptide spectra in addition to using bioinformatics tools to confirm the complexity of glycosylation in the spike protein. |
| T11 |
1426-1632 |
Sentence |
denotes |
The elucidation of the glycan repertoire on the spike protein provides insights into the viral binding studies and more importantly, propels research towards the development of a suitable vaccine candidate. |
