LitCovid-sentences  

PMC:7235801 / 7833-10711 JSONTXT 9 Projects

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Id Subject Object Predicate Lexical cue
T56 0-154 Sentence denotes The most widely used IVD assays for the confirmatory diagnosis of COVID-19 are based on RT-PCR that have been employed globally to deal with the pandemic.
T57 155-312 Sentence denotes A novel and robust real-time RT-PCR assay was developed by Tib-Molbiol, Germany, in collaboration with various partners by the 2nd week of January 2020 [14].
T58 313-404 Sentence denotes It was highly specific for SARS-CoV-2 RNA and did not cross-react with other coronaviruses.
T59 405-510 Sentence denotes The test detects the SARS-CoV-2 RNA via envelope (E) and RNA-dependent RNA polymerase (RdRp) gene assays.
T60 511-627 Sentence denotes The E-gene assay was used for first line screening, while the RdRp gene assay was employed for confirmatory testing.
T61 628-765 Sentence denotes In another approach, the researchers developed one-step RT-PCR assays to detect ORF1b and N regions of SARS-CoV-2 in 1 h and 15 min [15].
T62 766-849 Sentence denotes The N gene assay was used for screening, while Orf1b was used as confirmatory test.
T63 850-1031 Sentence denotes However, as the assay involved the detection of ORF1b and N regions that are highly conserved among sarbecoviruses, it could also bind to SARS-CoV and other closely-related viruses.
T64 1032-1190 Sentence denotes The authors mentioned that they could distinguish the SARS-CoV-2 from SARS-CoV via sequence analysis of positive amplicons if the RT-PCR results are positive.
T65 1191-1395 Sentence denotes Of interest was the development of real-time RT-PCR assay for the detection of RdRp/helicase (H) genes of SARS-CoV-2, which did not cross-react with other human coronaviruses and respiratory viruses [16].
T66 1396-1568 Sentence denotes Due to its high sensitivity, it can be used for COVID-19 detection in samples that have low viral loads and for testing saliva, plasma, and upper respiratory tract samples.
T67 1569-1660 Sentence denotes Many RT-PCR assays have already been developed by many companies and researchers worldwide.
T68 1661-1836 Sentence denotes The most prominent real-time RT-PCR rapid test is the Xpert® Xpress SARS-CoV-2 test by Cepheid, USA, which provides results in just 45 min using GenXpert benchtop system [17].
T69 1837-2025 Sentence denotes It is a rapid and automated point-of-care (POC) molecular test that enables the qualitative detection of SARS-CoV-2 in nasopharyngeal swab, nasal wash, or aspirate specimens from suspects.
T70 2026-2185 Sentence denotes The test requires only a minute for sample preparation, employs Cepheid’s Xpert® Xpress cartridge technology, and targets multiple regions of the viral genome.
T71 2186-2276 Sentence denotes It has also received Food and Drug Administration (FDA) emergency use authorization (EUA).
T72 2277-2424 Sentence denotes Another prominent advance is the Vivalytic COVID-19 test by Bosch, Germany [18], which detects SARS-CoV-2 infection in suspects in less than 2.5 h.
T73 2425-2503 Sentence denotes The test was developed by Bosch in collaboration with Randox Laboratories, UK.
T74 2504-2663 Sentence denotes It is a fully automated and rapid POC molecular test that can simultaneously detect SARS-CoV-2 and nine other respiratory viruses, including influenza A and B.
T75 2664-2806 Sentence denotes The sample is taken from the nose or throat of suspect using a swab and placed inside a Vivalytic cartridge containing all the assay reagents.
T76 2807-2878 Sentence denotes The cartridge is then plugged into a small benchtop Vivalytic analyzer.