PMC:7214329 / 6301-6913 7 Projects
Annnotations
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T45 | 0-40 | Sentence | denotes | Reverse transcription PCR and sequencing |
| T46 | 41-160 | Sentence | denotes | The suspension was used for real-time reverse transcription polymerase chain reaction (RT-PCR) assay of SARS-CoV-2 RNA. |
| T47 | 161-322 | Sentence | denotes | The real-time RT-PCR assay was performed using a SARS-CoV-2 nucleic acid detection kit according to the manufacturer's protocol (Shanghai ZJ Bio-Tech Co., Ltd.). |
| T48 | 323-612 | Sentence | denotes | Two different targets on the SARS-CoV-2 genome, namely, the RNA-dependent RNA polymerase (RdRp) and nucleocapsid (N) genes, were employed, and a third target, the envelope (E) gene, was used for real-time quantitative PCR.10 A sample was considered positive when the qPCR Ct value was ≤43. |