LitCovid-sentences  

PMC:7152911 / 67599-69255 JSONTXT 9 Projects

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Id Subject Object Predicate Lexical cue
T546 0-94 Sentence denotes Single-stranded DNA (ssDNA) is a useful biorecognition element for the detection of pathogens.
T547 95-263 Sentence denotes While ssDNA is commonly used as a biorecognition element for DNA-based assays, ssDNA aptamers are commonly used for pathogen detection using electrochemical biosensors.
T548 264-396 Sentence denotes Aptamers are single-stranded oligonucleotides capable of binding various molecules with high affinity and selectivity (Lakhin et al.
T549 397-420 Sentence denotes 2013; Reverdatto et al.
T550 421-427 Sentence denotes 2015).
T551 428-624 Sentence denotes Aptamers are isolated from a large random sequence pool through a selection process that utilizes systematic evolution of ligands by exponential enrichment, also known as SELEX (Stoltenburg et al.
T552 625-631 Sentence denotes 2007).
T553 632-760 Sentence denotes Suitable binding sequences can be isolated from a large random oligonucleotide sequence pool and subsequently amplified for use.
T554 761-842 Sentence denotes Thus, aptamers can exhibit high selectivity to target species (Stoltenburg et al.
T555 843-849 Sentence denotes 2007).
T556 850-957 Sentence denotes Aptamers can also be produced at a lower cost than alternative biorecognition elements, such as antibodies.
T557 958-1185 Sentence denotes Giamberardino et al. used SELEX to discover an aptamer for norovirus detection, which showed a million-fold higher binding affinity for the target than a random DNA strand that served as a negative control (Giamberardino et al.
T558 1186-1192 Sentence denotes 2013).
T559 1193-1344 Sentence denotes Iqbal et al. performed 10 rounds of SELEX to discover 14 aptamer clones with high affinities for C. parvum for detection in fruit samples (Iqbal et al.
T560 1345-1351 Sentence denotes 2015).
T561 1352-1649 Sentence denotes However, the use of aptamers as biorecognition elements has not yet replaced traditional biorecognition elements, such as antibodies, because of several challenges, such as aptamer stability, degradation, cross-reactivity, and reproducibility using alternative processing approaches (Lakhin et al.
T562 1650-1656 Sentence denotes 2013).