PMC:7152911 / 6125-8366 JSONTXT 9 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T51 0-108 Sentence denotes Over the past twenty-five years, biosensors have emerged to complement PCR and ELISA for pathogen detection.
T52 109-329 Sentence denotes Biosensors are based on the direct integration of a selective biorecognition element and a sensitive transducer element and provide complementary platforms to PCR and ELISA for pathogen identification and quantification.
T53 330-526 Sentence denotes According to the International Union of Pure and Applied Chemistry (IUPAC), a biosensor must contain a biorecognition element in direct spatial contact with a transduction element (Thévenot et al.
T54 527-533 Sentence denotes 2001).
T55 534-714 Sentence denotes In addition, a biosensor should provide quantitative or semi-quantitative analytical information and measurement without the requirement of additional processing steps or reagents.
T56 715-914 Sentence denotes While a biosensor should also be a self-contained, integrated device, the measurement approach can vary from droplet formats to continuous flow formats that require associated fluid handling systems.
T57 915-1061 Sentence denotes Biosensors have achieved sensitive and selective real-time detection of pathogens in various environments without the need for sample preparation.
T58 1062-1230 Sentence denotes For example, biosensors have enabled the detection of an abundance of pathogens in various matrices and environments, including foods, body fluids, and object surfaces.
T59 1231-1373 Sentence denotes In addition to sample preparation-free protocols, biosensors are compatible with label-free protocols (Daniels and Pourmand, 2007; Rapp et al.
T60 1374-1391 Sentence denotes 2010; Sang et al.
T61 1392-1416 Sentence denotes 2016; Vestergaard et al.
T62 1417-1423 Sentence denotes 2007).
T63 1424-1544 Sentence denotes Labels, often referred to as reporters, are molecular species, such as organic dyes or quantum dots (Resch-Genger et al.
T64 1545-1768 Sentence denotes 2008), that are attached to the target, either directly or through a biorecognition element, using a series of sample preparation steps or secondary binding steps to facilitate detection through the properties of the label.
T65 1769-1890 Sentence denotes Thus, label-free biosensors avoid the use of a reporter species to detect the target species (Cooper, 2009; Syahir et al.
T66 1891-1897 Sentence denotes 2015).
T67 1898-2234 Sentence denotes Label-free assays often have fewer sample preparation steps due to the elimination of procedures associated with target labeling and lower cost than label-based assays, which are important considerations for applications in which preparation facilities or trained personnel are either limited or unavailable (Cooper, 2009; Syahir et al.
T68 2235-2241 Sentence denotes 2015).