Id |
Subject |
Object |
Predicate |
Lexical cue |
T51 |
0-108 |
Sentence |
denotes |
Over the past twenty-five years, biosensors have emerged to complement PCR and ELISA for pathogen detection. |
T52 |
109-329 |
Sentence |
denotes |
Biosensors are based on the direct integration of a selective biorecognition element and a sensitive transducer element and provide complementary platforms to PCR and ELISA for pathogen identification and quantification. |
T53 |
330-526 |
Sentence |
denotes |
According to the International Union of Pure and Applied Chemistry (IUPAC), a biosensor must contain a biorecognition element in direct spatial contact with a transduction element (Thévenot et al. |
T54 |
527-533 |
Sentence |
denotes |
2001). |
T55 |
534-714 |
Sentence |
denotes |
In addition, a biosensor should provide quantitative or semi-quantitative analytical information and measurement without the requirement of additional processing steps or reagents. |
T56 |
715-914 |
Sentence |
denotes |
While a biosensor should also be a self-contained, integrated device, the measurement approach can vary from droplet formats to continuous flow formats that require associated fluid handling systems. |
T57 |
915-1061 |
Sentence |
denotes |
Biosensors have achieved sensitive and selective real-time detection of pathogens in various environments without the need for sample preparation. |
T58 |
1062-1230 |
Sentence |
denotes |
For example, biosensors have enabled the detection of an abundance of pathogens in various matrices and environments, including foods, body fluids, and object surfaces. |
T59 |
1231-1373 |
Sentence |
denotes |
In addition to sample preparation-free protocols, biosensors are compatible with label-free protocols (Daniels and Pourmand, 2007; Rapp et al. |
T60 |
1374-1391 |
Sentence |
denotes |
2010; Sang et al. |
T61 |
1392-1416 |
Sentence |
denotes |
2016; Vestergaard et al. |
T62 |
1417-1423 |
Sentence |
denotes |
2007). |
T63 |
1424-1544 |
Sentence |
denotes |
Labels, often referred to as reporters, are molecular species, such as organic dyes or quantum dots (Resch-Genger et al. |
T64 |
1545-1768 |
Sentence |
denotes |
2008), that are attached to the target, either directly or through a biorecognition element, using a series of sample preparation steps or secondary binding steps to facilitate detection through the properties of the label. |
T65 |
1769-1890 |
Sentence |
denotes |
Thus, label-free biosensors avoid the use of a reporter species to detect the target species (Cooper, 2009; Syahir et al. |
T66 |
1891-1897 |
Sentence |
denotes |
2015). |
T67 |
1898-2234 |
Sentence |
denotes |
Label-free assays often have fewer sample preparation steps due to the elimination of procedures associated with target labeling and lower cost than label-based assays, which are important considerations for applications in which preparation facilities or trained personnel are either limited or unavailable (Cooper, 2009; Syahir et al. |
T68 |
2235-2241 |
Sentence |
denotes |
2015). |