PMC:7152911 / 57880-59808 JSONTXT 9 Projects

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Id Subject Object Predicate Lexical cue
T465 0-280 Sentence denotes In addition to hybrid biosensor designs composed of combinations of electrodes with other transducers, hybrid biosensor-based assays for pathogen detection based on the combination of an electrochemical biosensor with a traditional bioanalytical technique have also been utilized.
T466 281-441 Sentence denotes For example, electrochemical-colorimetric (EC-C) biosensing combines an electrochemical method and a colorimetric, fluorescent, or luminescent detection method.
T467 442-662 Sentence denotes The electrode detects the presence of a target species, while the colorimetric transduction pathway enables quantification of the products associated with the reaction between the target and an active species (Hou et al.
T468 663-669 Sentence denotes 2018).
T469 670-875 Sentence denotes For example, Hou et al. used an EC-C approach based on a monoclonal antibody-functionalized AuNP-modified ITO electrode and dual-labeled magnetic beads for the detection of human enterovirus 71 (Hou et al.
T470 876-882 Sentence denotes 2018).
T471 883-1082 Sentence denotes In that study, antibody- and horseradish peroxidase (HRP)-labeled magnetic nanobeads were introduced as a secondary binding step following exposure of the electrode to enterovirus-containing samples.
T472 1083-1351 Sentence denotes Following the secondary binding step, the HRP-nanobead conjugates enabled colorimetric detection via monitoring of oxidative products produced by HRP-catalyzed redox reactions, while the functionalized electrode enabled electrochemical detection via chronoamperometry.
T473 1352-1474 Sentence denotes Various techniques often rely on the use of optically-active labels for colorimetric, fluorescent, or luminescent sensing.
T474 1475-1808 Sentence denotes The optical labels used in pathogen detection applications commonly include biological fluorophores, such as green fluorescent protein, non-protein organic fluorophores, such as fluorescein and rhodamine, and nanoparticles, such as quantum dots, including CdS, CdSe, and GaAs, among others (Mungroo and Neethirajan 2016; Pires et al.
T475 1809-1815 Sentence denotes 2014).
T476 1816-1928 Sentence denotes The use of such additional reagents to detect the target species is discussed further in the following sections.