Id |
Subject |
Object |
Predicate |
Lexical cue |
T465 |
0-280 |
Sentence |
denotes |
In addition to hybrid biosensor designs composed of combinations of electrodes with other transducers, hybrid biosensor-based assays for pathogen detection based on the combination of an electrochemical biosensor with a traditional bioanalytical technique have also been utilized. |
T466 |
281-441 |
Sentence |
denotes |
For example, electrochemical-colorimetric (EC-C) biosensing combines an electrochemical method and a colorimetric, fluorescent, or luminescent detection method. |
T467 |
442-662 |
Sentence |
denotes |
The electrode detects the presence of a target species, while the colorimetric transduction pathway enables quantification of the products associated with the reaction between the target and an active species (Hou et al. |
T468 |
663-669 |
Sentence |
denotes |
2018). |
T469 |
670-875 |
Sentence |
denotes |
For example, Hou et al. used an EC-C approach based on a monoclonal antibody-functionalized AuNP-modified ITO electrode and dual-labeled magnetic beads for the detection of human enterovirus 71 (Hou et al. |
T470 |
876-882 |
Sentence |
denotes |
2018). |
T471 |
883-1082 |
Sentence |
denotes |
In that study, antibody- and horseradish peroxidase (HRP)-labeled magnetic nanobeads were introduced as a secondary binding step following exposure of the electrode to enterovirus-containing samples. |
T472 |
1083-1351 |
Sentence |
denotes |
Following the secondary binding step, the HRP-nanobead conjugates enabled colorimetric detection via monitoring of oxidative products produced by HRP-catalyzed redox reactions, while the functionalized electrode enabled electrochemical detection via chronoamperometry. |
T473 |
1352-1474 |
Sentence |
denotes |
Various techniques often rely on the use of optically-active labels for colorimetric, fluorescent, or luminescent sensing. |
T474 |
1475-1808 |
Sentence |
denotes |
The optical labels used in pathogen detection applications commonly include biological fluorophores, such as green fluorescent protein, non-protein organic fluorophores, such as fluorescein and rhodamine, and nanoparticles, such as quantum dots, including CdS, CdSe, and GaAs, among others (Mungroo and Neethirajan 2016; Pires et al. |
T475 |
1809-1815 |
Sentence |
denotes |
2014). |
T476 |
1816-1928 |
Sentence |
denotes |
The use of such additional reagents to detect the target species is discussed further in the following sections. |