| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T81 |
0-353 |
Sentence |
denotes |
The work presented at the 4th Microfluidic Handling Systems conference and which is extended in this paper aims at the development of a disposable, polymer-based DNA amplification lab-on-chip system with integrated resistive heater based on the World Health Organization (WHO) Sexually Transmitted Diseases Diagnostics Initiative (SDI) ASSURED criteria. |
| T82 |
354-541 |
Sentence |
denotes |
Devices which are ASSURED are (A) affordable, (S) sensitive, (S) specific, (U) user-friendly, (R) robust and rapid, (E) equipment-free, and (D) deliverable to those who need them [20,49]. |
| T83 |
542-617 |
Sentence |
denotes |
The first step towards such a device is the development of the chip itself. |
| T84 |
618-717 |
Sentence |
denotes |
This paper focuses on the choice of substrate material, metal deposition method, and type of metal. |
| T85 |
718-875 |
Sentence |
denotes |
Although, it is mentioned above that PCR and HDA are sequence specific, the reaction chosen is the isothermal multiple displacement amplification (MDA) [50]. |
| T86 |
876-1154 |
Sentence |
denotes |
This reaction is more straightforward [51], as it amplifies any present DNA, and is therefore better suitable as a proof-of-principle amplification reaction to show the functioning of the integrated heater and the biocompatibility of the substrate after the fabrication process. |
| T87 |
1155-1294 |
Sentence |
denotes |
The use of an isothermal amplification technique also simplifies the final device and lowers its footprint, as there are no pumps required. |
| T88 |
1295-1450 |
Sentence |
denotes |
In this research, external analysis methods are used which do not contribute to the WHO-SDI ASSURED criteria due to their bulkiness, costs, and difficulty. |
| T89 |
1451-1577 |
Sentence |
denotes |
However, suggestions and comments on the integration of low-cost detection methods, which are ASSURED, are given in Section 5. |
