| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T342 |
0-125 |
Sentence |
denotes |
With the proof-of-principle device, successful DNA amplifications using MDA inside a disposable polymeric chip were achieved. |
| T343 |
126-214 |
Sentence |
denotes |
The heat for the reaction was applied using the integrated low-cost Au-resistive heater. |
| T344 |
215-384 |
Sentence |
denotes |
The device was operated at a suitable temperature for MDA reactions and the amplified DNA was measured using EvaGreen fluorescence dye and an ex situ spectrofluorometer. |
| T345 |
385-473 |
Sentence |
denotes |
A distinct peak is visible in the reaction mixtures which is absent in the NTC mixtures. |
| T346 |
474-578 |
Sentence |
denotes |
The operating temperature for MDA reactions is around 30 °C, which is comparable with a nice summer day. |
| T347 |
579-691 |
Sentence |
denotes |
Using amplification reactions which such low reaction temperatures could encounter problems at warmer locations. |
| T348 |
692-794 |
Sentence |
denotes |
However, as MDA is not sequence specific, this reaction will not be integrated in the final protocols. |
| T349 |
795-928 |
Sentence |
denotes |
MDA was only used as proof-of-principle reaction to show the biocompatibility of the device and functioning of the integrated heater. |
| T350 |
929-1055 |
Sentence |
denotes |
Sequence specific amplifications, e.g., HDA and LAMP, are performed at higher temperatures, as will be discussed in Section 5. |
| T351 |
1056-1116 |
Sentence |
denotes |
This makes the system less sensitive to the hot summer days. |
