| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T321 |
0-190 |
Sentence |
denotes |
After the amplifications, the reaction mixtures are pipetted out of the chips and tubes and into 1 mL quartz cuvettes containing 55 μL MilliQ DI water (Merck Millipore, Burlington, MA, USA). |
| T322 |
191-308 |
Sentence |
denotes |
Fluorescence measurements are done in a Horiba Scientific FluoroMax+ spectrofluorometer to verify each amplification. |
| T323 |
309-484 |
Sentence |
denotes |
The mixture is excitated at a wavelength of 500 nm and the emission spectrum is measured at wavelengths from 510 nm to 550 nm (bounded EvaGreen dye has a peak at 525 nm [63]). |
| T324 |
485-645 |
Sentence |
denotes |
The measured spectra are normalized by subtracting the background signal of a mixture containing only the reaction buffer, the sample buffer, EvaGreen, and DNA. |
| T325 |
646-727 |
Sentence |
denotes |
No Enzyme was added to this mixture, therefore no amplification could take place. |
| T326 |
728-799 |
Sentence |
denotes |
See Figure 15 for the results obtained in the Eppendorf tube and chips. |
| T327 |
800-894 |
Sentence |
denotes |
Figure A4 in Appendix E shows the background signal which is subtracted from all measurements. |
