| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T305 |
0-202 |
Sentence |
denotes |
MDA reactions are also performed inside an Eppendorf tube (Eppendorf AG, Hamburg, Germany) and inside the chip, again using the Illustra GenomiPhi V2 DNA amplification kit and EvaGreen fluorescence dye. |
| T306 |
203-317 |
Sentence |
denotes |
As heat supply the water bath of an IKA Rotary Evaporator RV 8V (IKA-Werke, Staufen im Breisgau, Germany) is used. |
| T307 |
318-421 |
Sentence |
denotes |
This water bath is according to its specification stable within a range of the set temperature ±0.1 °C. |
| T308 |
422-546 |
Sentence |
denotes |
The chip and an Eppendorf tube are loaded with the reaction mixture containing the DNA sample and the EvaGreen dye solution. |
| T309 |
547-680 |
Sentence |
denotes |
Here, the Eppendorf tube is serving as a control to show that the fabrication steps of the chips are not inhibiting the MDA reaction. |
| T310 |
681-771 |
Sentence |
denotes |
The inlet and outlet of the chip are sealed with the Microseal “B” PCR plate sealing foil. |
| T311 |
772-908 |
Sentence |
denotes |
The closed chip and tube are heated up in a separate water bath to 95 °C and kept at that temperature for 3 min to denaturate the dsDNA. |
| T312 |
909-1043 |
Sentence |
denotes |
Subsequently, the chip and tube are cooled down by placing it in an ice bath for 5 min after which the rest of the reagents are added. |
| T313 |
1044-1106 |
Sentence |
denotes |
The complete mixtures are according to Table A3 in Appendix D. |
| T314 |
1107-1280 |
Sentence |
denotes |
After closing the chip and tube again, they are placed in the water bath of the rotary evaporater and left there for 90 min, after which the reaction is terminated at 65 °C. |
