| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T300 |
0-188 |
Sentence |
denotes |
First, to determine the temperature window of operation, MDA reactions are performed at 25 °C and 30 °C using the Illustra GenomiPhi V2 DNA amplification kit and EvaGreen fluorescence dye. |
| T301 |
189-338 |
Sentence |
denotes |
From the literature, we know that this reaction does not work above 35 °C due to degradation of the protein activity in presence of a substrate [44]. |
| T302 |
339-485 |
Sentence |
denotes |
In Figure 13, a graph of the fluorescence signal during MDA reactions at 25 °C and 30 °C, together with their non template control (NTC) is shown. |
| T303 |
486-861 |
Sentence |
denotes |
These reactions are carried out in a conventional Bio-Rad CFX96 Touch Real-Time PCR machine (Bio-Rad Laboratories, Inc., Hercules, CA, USA) and the results show that the chosen proof-of-principle DNA amplification reaction is temperature dependent to some extent, but that there is a wide range of temperatures at which the amplification can be performed, i.e., 25°C to 35°C. |
| T304 |
862-977 |
Sentence |
denotes |
This makes the functioning of the integrated resistive heater less critical than the stability shown in Figure 11c. |
