PMC:7143804 / 30619-33004 JSONTXT 7 Projects

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Id Subject Object Predicate Lexical cue
T236 0-32 Sentence denotes Milling and Optical Transparency
T237 33-127 Sentence denotes The milling increased the surface roughness of the COC plates also increases the surface area.
T238 128-267 Sentence denotes Inhibition of the amplification can be caused by large surface areas as the used chemicals have more surface to have interaction with [44].
T239 268-606 Sentence denotes The created surface roughness is visualized using a FEI Sirion high resolution scanning electron microscope (HR-SEM) (FEI Company, Hillsboro, OR, USA) and measured using a Bruker Icon Dimension AFM in tapping mode with Bruker Tespa-V2 cantilevers (Bruker Nano Surfaces, Santa Barbara, CA, USA) and Gwyddion 2.52 open source freeware [62].
T240 607-641 Sentence denotes The results are shown in Figure 8.
T241 642-701 Sentence denotes The surface roughness of pristine COC had a RRMS of 3.5 nm.
T242 702-774 Sentence denotes This increased two orders of magnitude after milling (RRMS of 310.1 nm).
T243 775-974 Sentence denotes With the reported surface treatment [44,59] we were capable of decreasing the surface roughness to a value even lower than that of pristine COC and the lowest reported in literature (RRMS of 0.9 nm).
T244 975-1186 Sentence denotes For this grade of COC (TOPAS 6017) it worked the best to do four short exposures of 5 s, with N2 blow drying after each exposure, instead of one longer exposure, as is more common in other grades of COC [44,59].
T245 1187-1336 Sentence denotes The difference in duration for the cyclohexane vapor post-treatment can be explained by the different ratios of the copolymers present in each grade.
T246 1337-1450 Sentence denotes As the grade number increase, the ratio changes towards more norbornene monomers and less linear ethene monomers.
T247 1451-1638 Sentence denotes The norbornene is more apolar due to the bridged cyclic hydrocarbon present in its molecular structure and therefore, will dissolve faster in non-polar solvents, like cyclohexane (vapor).
T248 1639-1720 Sentence denotes Lowering the surface roughness also increased the optical transmittance fivefold.
T249 1721-1821 Sentence denotes Transmittance measurements in the visible range are done using a Woollam M-2000UI ellipsometer (J.A.
T250 1822-1853 Sentence denotes Woollam Co., Lincoln, NE, USA).
T251 1854-1904 Sentence denotes The results can be seen in Figure 9 and Figure 10.
T252 1905-2144 Sentence denotes Having a high optical transparency in the visible range can be desired when in situ fluorescence detection will be implemented (e.g., EvaGreen fluorescence dye has an excitation wavelength of 500 nm and emission wavelength of 525 nm [63]).
T253 2145-2385 Sentence denotes However, as in situ fluorescence detection is not used yet in this system and can also be done through the transparent PCR plate sealing foil, no further effort is put into optimizing this procedure to get even better optical transmittance.