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PMC:7143804 / 13482-15854 JSONTXT 8 Projects

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Id Subject Object Predicate Lexical cue
T112 0-4 Sentence denotes 2.1.
T113 5-34 Sentence denotes Microfluidic Structure Design
T114 35-147 Sentence denotes The microfluidic structure consists of two chambers, i.e., a reaction chamber and a temperature monitor chamber.
T115 148-199 Sentence denotes In Figure 2, a close-up of the final chip is shown.
T116 200-288 Sentence denotes For clarity reasons, the two microfluidic structures are colored with food coloring dye.
T117 289-737 Sentence denotes The reaction chamber is based on the work of Bruijns et al. [36] and its dimensions are chosen in such way that the internal volume of the reaction chamber is the same as the reaction volume of the used Illustra GenomiPhi V2 DNA amplification kit (GE Healthcare Life Sciences, Eindhoven, The Netherlands) together with the EvaGreen dye solution (Biotium, Fremont, CA, USA), while maintaining an as low as possible surface-area-to-volume ratio [44].
T118 738-1083 Sentence denotes Using SolidWorks 2018 computer-aided design (CAD) software (Dassault Systemes, Vélizy-Villacoublay, France), the 3D image of the chip is drawn and with the use of the AutoDesk HSMWorks computer-aided manufacturing (CAM) plug-in (Autodesk Inc., San Rafael, CA, USA), this image is transferred into a computer numerical control (CNC) milling code.
T119 1084-1172 Sentence denotes The total chip size is 3 cm by 3 cm and contains an inlet and outlet of 1.5 mm diameter.
T120 1173-1270 Sentence denotes The inlet and outlet are of such size that the reaction chamber can be filled using pipette tips.
T121 1271-1363 Sentence denotes In between the inlet and outlet, a rectangular reaction chamber of 10 mm by 3 mm is located.
T122 1364-1465 Sentence denotes Two trapezoid structures are placed in the tapered channels between the inlet/outlet and the chamber.
T123 1466-1510 Sentence denotes The function of these trapezoids is twofold:
T124 1511-1671 Sentence denotes First, they minimize the dead volume between the inlet/outlet and the reaction chamber, locating as much as possible of the reaction mixture inside the chamber.
T125 1672-1725 Sentence denotes Second, they provide support for the chamber closure.
T126 1726-1883 Sentence denotes A stadium-shaped channel of 1.5 mm wide and 1.0 mm deep is located next to the reaction chamber, in such way that this channel is also covered by the heater.
T127 1884-1935 Sentence denotes This channel serves as temperature monitor chamber.
T128 1936-2039 Sentence denotes A thermocouple is inserted in this channel for real-time monitoring of the temperature inside the chip.
T129 2040-2134 Sentence denotes This way, a more accurate temperature of the reaction mixture inside the chip can be obtained.
T130 2135-2205 Sentence denotes Via a feedback loop, the input potential can be changed when required.
T131 2206-2295 Sentence denotes In Figure 3, the SolidWorks design of the chamber-based chip with both chambers is shown.
T132 2296-2372 Sentence denotes In Figure A1, in Appendix A, the technical drawing of the chip can be found.