| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T106 |
0-244 |
Sentence |
denotes |
Even though MDA is considered an isothermal process, prior to the reaction and to the addition of most reactants, the dsDNA and a buffer are heated up to 95 °C to denature the dsDNA to ssDNA and to give hexamers the initial access to the ssDNA. |
| T107 |
245-325 |
Sentence |
denotes |
After the amplification reaction, the polymerase has to be inactivated at 65 °C. |
| T108 |
326-469 |
Sentence |
denotes |
However, this does not require fast temperature changes, as would be the case with, for example, the temperature cycling in PCR amplifications. |
| T109 |
470-686 |
Sentence |
denotes |
This, together with the robustness of the amplification (it is a self-limiting reaction that amplifies all present DNA [50]) makes MDA perfectly suitable as proof-of-principle amplification reaction for such devices. |
