LitCovid-sentences  

PMC:7118659 / 18438-21905 JSONTXT 15 Projects

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Id Subject Object Predicate Lexical cue
T121 151-413 Sentence denotes SARS-CoV2, like other human coronaviruses, harbours three envelope proteins, the spike (S) protein (180–220 kDa), the membrane (M) protein (25–35 kDa) and the envelope (E) protein (10–12 kDa), which are required for entry of infectious virions into target cells.
T122 414-553 Sentence denotes The virion also contains the nucleocapsid (N), capable of binding to viral genomic RNA, and nsp3, a key component of the replicase complex.
T123 554-678 Sentence denotes A subset of betacoronaviruses use a hemagglutinin-esterase (65 kDa) that binds sialic acids at the surface of glycoproteins.
T124 679-726 Sentence denotes The S glycoprotein determines the host tropism.
T125 727-844 Sentence denotes There is indication that SARS-CoV-2 binds to angiotensin-converting enzyme 2 (ACE2) expressed on pneumocytes [85,99].
T126 845-1117 Sentence denotes Binding to ACE2 is expected to trigger conformational changes in the S glycoprotein allowing cleavage by the transmembrane protease TMPRSS2 of the S protein and the release of S fragments into the cellular supernatant that inhibit virus neutralisation by antibodies [100].
T127 1118-1426 Sentence denotes The virus is then transported into the cell through the early and late endosomes where the host protease cathepsin L further cleaves the S protein at low pH, leading to fusion of the viral envelope and phospholipidic membrane of the endosomes resulting in release of the viral genome into the cell cytoplasm.
T128 1427-1594 Sentence denotes Replication then starts and the positive-strand viral genomic RNA is transcribed into a negative RNA strand that is used as a template for the synthesis of viral mRNA.
T129 1595-1701 Sentence denotes Synthesis of the negative RNA strand peaks earlier and falls faster than synthesis of the positive strand.
T130 1702-1794 Sentence denotes Infected cells contain between 10 and 100 times more positive strands than negative strands.
T131 1795-2086 Sentence denotes The ribosome machinery of the infected cells is diverted in favour of the virus, which then synthesises its non-structural proteins (NSPs) that assemble into the replicase-transcriptase complex to favour viral subgenomic mRNA synthesis (see the review by Fehr and Perlman for details [101]).
T132 2087-2230 Sentence denotes Following replication, the envelope proteins are translated and inserted into the endoplasmic reticulum and then move to the Golgi compartment.
T133 2231-2374 Sentence denotes Viral genomic RNA is packaged into the nucleocapsid and then envelope proteins are incorporated during the budding step to form mature virions.
T134 2375-2532 Sentence denotes The M protein, which localises to the trans-Golgi network, plays an essential role during viral assembly by interacting with the other proteins of the virus.
T135 2533-2665 Sentence denotes Following assembly, the newly formed viral particles are transported to the cell surface in vesicles and are released by exocytosis.
T136 2666-2794 Sentence denotes It is possible that chloroquine interferes with ACE2 receptor glycosylation, thus preventing SARS-CoV-2 binding to target cells.
T137 2795-2926 Sentence denotes Chloroquine could also possibly limit the biosynthesis of sialic acids that may be required for cell surface binding of SARS-CoV-2.
T138 2927-3081 Sentence denotes If binding of some viral particles is achieved, chloroquine may modulate the acidification of endosomes thereby inhibiting formation of the autophagosome.
T139 3082-3210 Sentence denotes Through reduction of cellular mitogen-activated protein (MAP) kinase activation, chloroquine may also inhibit virus replication.
T140 3211-3313 Sentence denotes Moreover, chloroquine could alter M protein maturation and interfere with virion assembly and budding.
T141 3314-3425 Sentence denotes With respect to the effect of chloroquine on the immune system, see the elegant review by Savarino et al. [11].
T142 3426-3467 Sentence denotes ERGIC, ER-Golgi intermediate compartment.