LitCovid-sentences  

PMC:7115396 / 8851-10593 JSONTXT 7 Projects

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Id Subject Object Predicate Lexical cue
T53 0-111 Sentence denotes The rIBV rH120-S1/YZ strain used in this study is described in the schematic illustration presented in Fig. 1 .
T54 112-243 Sentence denotes The genome RNA was synthesized in vitro by T7 RNA polymerase and transfected into BHK-21 cells, as previously described [35], [36].
T55 244-358 Sentence denotes The cell supernatants were harvested 48 h following transfection and propagated in 10-day-old SPF chicken embryos.
T56 359-478 Sentence denotes The allantoic fluid was harvested for RT-PCR, whole genome sequencing, and was then stored at −80 °C until further use.
T57 479-711 Sentence denotes Fig. 1 Schematic diagram for the construction of the chimeric S gene and production of a full-length cDNA of rH120-S1/YZ. (A) Replacement of the H120 S1 fragment by the corresponding sequence of IBYZ for construction of pYZS1H120S2.
T58 712-878 Sentence denotes The plasmids pIBYZS and pH120S contained the S gene of IBYZ strain and H120 strain, respectively, were constructed during the establishment of reverse genetic system.
T59 879-1047 Sentence denotes Primers PS1F and PS1R were used to amplify the S1 fragment of IBYZ and vector fragment, while primers PS2F and PS2R were used to amplify the S2 fragment of H120 strain.
T60 1048-1211 Sentence denotes By overlapping PCR, the pYZS1H120S2 was constructed which contained a chimeric S gene. (B) Strategy for the construction of full-length cDNA clones of rH120-S1/YZ.
T61 1212-1297 Sentence denotes Ten cDNA fragments covering the entire genome of H120 strain was amplified by RT-PCR.
T62 1298-1500 Sentence denotes Unique Bsa I sites were inserted at the junctions between each clone, a unique T7 start site was inserted at the 5′ end of clone TM1, and a 28-nucleotide T tail was inserted at the 3′ end of clone TM10.
T63 1501-1585 Sentence denotes The S fragment in the pMDTM8 plasmid of H120 strain was replaced by chimeric S gene.
T64 1586-1742 Sentence denotes By using appropriate ligation strategy, the genomic cDNA of rH120-S1/YZ was assembled by in vitro ligation using appropriate restriction sites as indicated.