| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T37 |
0-212 |
Sentence |
denotes |
In considering the limitations of the live attenuated and inactivated IBV vaccines, reverse genetic IBV vaccines have been developed in recent years as they display increased safety and efficacy [28], [29], [30]. |
| T38 |
213-524 |
Sentence |
denotes |
As reverse genetic technology is primarily based on the H120 and Beaudette strains [31], [32], [33], both of which are passage-generated attenuated strains, the mechanism of virus virulence attenuation remains unclear, thereby posing difficulties in the development of attenuated vaccines using this technology. |
| T39 |
525-688 |
Sentence |
denotes |
In addition to the function of host invasion and increasing genetic diversity, the IBV S1 subunit can induce virus neutralizing and cross-reactive antibodies [34]. |
| T40 |
689-933 |
Sentence |
denotes |
Therefore, some researchers have created an attenuated IBV vaccine by replacing the S1 or S genes from other virulent strains using reverse genetic technology to generate protection against strains of the corresponding serotype [5], [28], [30]. |
| T41 |
934-1216 |
Sentence |
denotes |
In the present study, we constructed a recombinant strain, using the reverse genetic system. rH120-S1/YZ is based on the backbone of the vaccine strain H120, and the S1 gene was replaced with that of the QX-like nephropathogenic strain, ck/CH/IBYZ/2011, which was isolated in China. |
| T42 |
1217-1360 |
Sentence |
denotes |
Our results demonstrate that this recombinant strain is safe and provides effective protection in young chickens against QX-like IBV challenge. |
