| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T220 |
0-76 |
Sentence |
denotes |
In the present study, we isolated a nephoropathogenic strain (IBYZ) in 2011. |
| T221 |
77-214 |
Sentence |
denotes |
This strain was isolated, identified, and preserved from a large-scale chicken farm where an outbreak of IBV emerged in Jiangsu province. |
| T222 |
215-318 |
Sentence |
denotes |
Chickens infected with the IBYZ strain showed severe kidney damage and a high mortality rate (Fig. 3B). |
| T223 |
319-423 |
Sentence |
denotes |
The complete sequence has been deposited in the GenBank database under the accession number of KF663561. |
| T224 |
424-642 |
Sentence |
denotes |
The S1 gene sequencing data indicated that the IBYZ strain belonged to the QX-like genotype (Fig. 8 ), with a nucleotide homology with the attenuated vaccine H120 strain of only 77.4%, and amino acid homology of 77.1%. |
| T225 |
643-752 |
Sentence |
denotes |
Moreover, the S1/S2 cleavage site of IBYZ is HRRRR/S, which differed from RRFRR/S of the H120 vaccine strain. |
| T226 |
753-871 |
Sentence |
denotes |
After whole-genome sequencing, we constructed a molecular clone strain using reverse genetic technology, termed rIBYZ. |
| T227 |
872-1044 |
Sentence |
denotes |
In addition, another strain used in this study is the live attenuated IBV vaccine, H120, and the molecular clone strain, rH120, which was constructed using the same method. |
| T228 |
1045-1216 |
Sentence |
denotes |
Fig. 8 Phylogenetic tree constructed based on the S1 gene of isolate ck/CH/IBYZ/2011 and 28 published IBV reference strains using the neighbor-joining method (Mega10.1.6). |
