LitCovid-sentences  

PMC:7115396 / 21240-27709 JSONTXT 10 Projects

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Id Subject Object Predicate Lexical cue
T148 0-11 Sentence denotes 3.2 Safety
T149 13-81 Sentence denotes 3.2.1 Pathogenicity of the recombinants in one-day-old SPF chickens
T150 82-276 Sentence denotes The earliest death was observed at 3 dpi in the QX-like strain rIBYZ group, which virus was highly pathogenic to one-day-old SPF chickens; the final mortality of this group was 63.3% (Fig. 3 A).
T151 277-589 Sentence denotes The morbidity of the infected chickens in the rIBYZ group reached higher than 80%, and the diseased chickens exhibited respiratory symptoms (e.g., sneezing, coughing, as well as tracheal and bronchiolar rales), and the severe cases presented with additional signs of listlessness, huddling, and ruffled feathers.
T152 590-783 Sentence denotes At necropsy, lesions were detected both in the respiratory and urinary system, including mucus, hyperemia, and hemorrhage in the trachea, as well as swelling and urate deposition in the kidney.
T153 784-858 Sentence denotes Edema and congestion were observed in the lungs in 20% of cases (Fig. 3B).
T154 859-1052 Sentence denotes However, following inoculation with the rH120 or rH120-S1/YZ strains, the morbidity rates of the chickens was less than 20%, with moderate respiratory signs of coughing in some of the chickens.
T155 1053-1228 Sentence denotes All of the infected chickens presented no visible lesions in the kidney and the survival rate was 100% through 14 dpi in both the rH120 and rH120-S1/YZ groups (Fig. 3A and B).
T156 1229-1334 Sentence denotes No obvious clinical signs, gross lesions, or death attributable to IBV was observed in the control group.
T157 1335-1506 Sentence denotes Fig. 3 Pathogenicity of the rIBV rH120, rIBYZ, and rH120-S1/YZ strains. (A) Percent survival of SPF chickens infected at 1-day-of age during the 14-day observation period.
T158 1507-1661 Sentence denotes No mortality was observed in rH120, rH120-S1/YZ, or the control groups. (B) Gross lesions on the lungs and kidneys of the infected chickens at 5 or 7 dpi.
T159 1663-1686 Sentence denotes 3.2.2 Tissue pathology
T160 1687-1902 Sentence denotes For the chickens inoculated with the QX-like strain rIBYZ, lesions in the trachea at 5 dpi were characterized as cilia necrosis and loss, epithelial cell congestion, hemorrhage, and lymphoid infiltration (Fig. 4 C).
T161 1903-2108 Sentence denotes In the bronchial and capillary lumen of rIBYZ-infected lungs, congestion, hemorrhage, as well as erythrocyte and inflammatory cell infiltration was observed in some severe cases from 7 to 10 dpi (Fig. 4G).
T162 2109-2254 Sentence denotes Moreover, erythrocyte and lymphocyte infiltration, as well as tubular dilation was observed in the kidney of rIBYZ-inoculated chickens (Fig. 4K).
T163 2255-2412 Sentence denotes Similar to the rH120 group, chickens inoculated with rH120-S1/YZ presented with mild pathology in the trachea at the early infection time points of 3, 5 dpi.
T164 2413-2571 Sentence denotes Lesions in the trachea consisted of the loss of epithelial cells and cilia, decreased number of secretory cells, and thickening of the mucosa (Fig. 4B and D).
T165 2572-2734 Sentence denotes In contrast, there were no lesions observed in the lungs and kidneys of rH120 and rH120-S1/YZ-infected chickens during the infection period (Fig. F, H, J, and L).
T166 2735-2927 Sentence denotes No histopathological evidence of damage was detected in the bursa of all the groups and no IBV-associated lesions were found in the tissue samples of the control chickens (Fig. 4A, E, I, M–P).
T167 2928-3103 Sentence denotes Fig. 4 Histopathological changes in the trachea, lungs, kidneys, and bursa of SPF chickens infected with the recombinant rH120, rIBYZ, and rH120-S1/YZ strains at 1-day-of-age.
T168 3104-3165 Sentence denotes Black arrows in the trachea indicate cilia and necrosis loss.
T169 3166-3227 Sentence denotes Black arrows in the lungs indicate congestion and hemorrhage.
T170 3228-3393 Sentence denotes Trachea samples were collected at 3, 5 dpi (A–D); lung and bursa samples were collected at 7, 10 dpi (E–H; M–P); and kidney samples were collected at 5, 7 dpi (I–L).
T171 3394-3474 Sentence denotes Black arrows in kidneys indicate tubular dilation, congestion, and inflammation.
T172 3475-3504 Sentence denotes Scale bar = 100 μm or 200 μm.
T173 3506-3557 Sentence denotes 3.2.3 Growth in tracheal, lung, and kidney tissues
T174 3558-3735 Sentence denotes The time-dependent levels of viral replication in the different tissues determined by real-time RT-PCR presented in Fig. 5 are consistent with the tissue histopathology results.
T175 3736-3919 Sentence denotes Compared to rH120 and rH120-S1/YZ groups, one day after inoculation with the QX-like strain, rIBYZ, the viral load in all organs began to rise rapidly, except at 5 dpi in the trachea.
T176 3920-4010 Sentence denotes The viral load in tracheal samples in the rH120-S1/YZ group increased and peaked at 5 dpi.
T177 4011-4113 Sentence denotes The level of viral RNA was significantly lower than the rH120 group both at 1 dpi and 7 dpi (Fig. 5A).
T178 4114-4336 Sentence denotes The viral load in lung samples from all infection groups increased starting at 3 dpi, and the viral RNA in the lungs of the rH120-S1/YZ group was significantly lower compared with both the rH120 and rIBYZ groups (Fig. 5B).
T179 4337-4417 Sentence denotes The viral load in the kidney of all IBV-inoculated groups was detected at 1 dpi.
T180 4418-4594 Sentence denotes In contrast to the rH120 and rH120-S1/YZ groups, the viral growth in kidney of rIBYZ-infected chickens exhibited an extremely significant increase at 3 dpi and peaked at 5 dpi.
T181 4595-4705 Sentence denotes The viral RNA copies of the rH120-S1/YZ group peaked at 1 dpi, after which they gradually decreased (Fig. 5C).
T182 4706-4782 Sentence denotes No viral load was detected in the control samples at any of the time points.
T183 4783-4947 Sentence denotes Fig. 5 Viral load in the different organs at 1, 3, 5, and 7 dpi of SPF chickens infected with the recombinant strains rH120, rIBYZ, and rH120-S1/YZ at 1-day-of age.
T184 4948-5178 Sentence denotes The bars indicate means ± standard deviations. ***Indicates an extremely significant difference at P ≤ 0.001; **Indicates a highly significant difference at 0.001 < P ≤ 0.01; *Indicates a significant difference at 0.01 < P ≤ 0.05.
T185 5180-5227 Sentence denotes 3.2.4 Viral load in the oral and cloacal swabs
T186 5228-5363 Sentence denotes In all IBV infection groups, the viral load in both the oral and cloacal swabs generally showed a gradual decline from 7 dpi to 26 dpi.
T187 5364-5704 Sentence denotes Compared with the rH120 group, The reduction in viral load between 14 or 18 dpi was significantly greater for chickens infected with rH120-S1/YZ than for chickens infected with rH120, and the viral RNA of the QX-like strain rIBYZ was lower than that of the rH120 group at 7, 14, and 18 dpi but with no statistically significance (Fig. 6 A).
T188 5705-5852 Sentence denotes In contrast, the viral load in the cloacal swab of the rIBYZ group was significantly higher than that of the rH120 and rH120-S1/YZ groups at 7 dpi.
T189 5853-5995 Sentence denotes Compared with other groups, the viral RNA in the cloacal swab of the rH120-S1/YZ group was significantly lower at 7, 14, and 22 dpi (Fig. 6B).
T190 5996-6057 Sentence denotes No virus was detected in the control group at any time point.
T191 6058-6216 Sentence denotes Fig. 6 Viral loads at 7, 14, 18, 22, and 26 dpi in the oral and cloacal swabs of chickens infected with the recombinant rH120, rIBYZ, and rH120-S1/YZ strains.
T192 6217-6469 Sentence denotes All data are presented as the mean ± standard error of the mean (SEM); ***Indicates an extremely significant difference at P ≤ 0.001; **Indicates a highly significant difference at 0.001 < P ≤ 0.01; *Indicates significant difference at 0.01 < P ≤ 0.05.