LitCovid-sentences  

PMC:7115396 / 16954-18685 JSONTXT 8 Projects

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Id Subject Object Predicate Lexical cue
T115 0-40 Sentence denotes 2.8 Efficacy of rH120-S1/YZ vaccination
T116 42-64 Sentence denotes 2.8.1 Viral challenge
T117 65-149 Sentence denotes In total, 30 one-day-old SPF chickens were divided into three groups of 10 chickens.
T118 150-301 Sentence denotes The three groups were intranasally vaccinated with the rH120 and rH120-S1/YZ strains at a dose of 104 EID50/200 μL, and the control group received PBS.
T119 302-463 Sentence denotes Two weeks post-vaccination, the chickens in each group were challenged with the QX-like strain, rIBYZ, at 106 EID50/200 μL via eye drop and the intranasal route.
T120 464-563 Sentence denotes Any clinical signs, as well as the percentage of morbidity and mortality were recorded for 14 days.
T121 564-646 Sentence denotes The dead chickens were also examined for gross tracheal, lung, and kidney lesions.
T122 648-729 Sentence denotes 2.8.2 Antibody response measured by an enzyme-linked immunosorbent assay (ELISA)
T123 730-806 Sentence denotes 45 1-day-old SPF chickens were divided into three groups (n = 15 per group).
T124 807-1066 Sentence denotes After vaccinating with the rH120 and rH120-S1/YZ strains at a dose of 104 EID50/200 μL, the sera of 10 birds from each group were randomly collected and antibodies were tested using an ELISA created in our lab at 7, 14, 21, and 28 days post-vaccination (dpv).
T125 1067-1240 Sentence denotes ELISA plates were coated with antigen of culture supernatants of inactivated IBV rMJ , a vero cells adaption strain domesticated from its parental strain of IBYZ in our lab.
T126 1241-1376 Sentence denotes Individual chicken sera collected from different groups diluted to 1:200 were loaded and the plates were incubated at 37 °C for 60 min.
T127 1377-1519 Sentence denotes IBV-specific IgG was detected with anti-chicken IgY (IgG) (whole molecule)-peroxidase antibodies produced in rabbits (Sigma-Aldrich, Germany).
T128 1520-1731 Sentence denotes After incubated with 3,3′,5,5′-Tetramethylbenzidine(TMB) substrate for 15 min and terminated with 2 mol/L H2SO4 solution, the absorbance at 490 nm was measured using a Model 680 microplate reader (Bio-Rad, USA).