PMC:7115396 / 16232-16952 JSONTXT 9 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T110 0-21 Sentence denotes 2.7.4 Viral shedding
T111 22-236 Sentence denotes To determine the level of viral shedding of the different recombinants, 10 birds per group were inoculated with 200 μL allantoic fluid containing 105 EID50 of the rH120, rIBYZ, or rH120-S1/YZ strains, respectively.
T112 237-336 Sentence denotes 200 μL PBS per chick was administered to the control group via eye drop and the intranasal route. .
T113 337-458 Sentence denotes Oral and cloacal swabs were collected from each bird into 1 mL PBS at 7, 14, 18, 22, 26, and 30 dpi and stored at −80 °C.
T114 459-720 Sentence denotes After freezing and thawing three times and centrifuging at 8,000 × g for 5 min at 4 °C, relative amount of virus present in 200 μL of supernatants of each sample was quantified by RNA extraction, reverse transcription, and real-time PCR as described previously.