| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T98 |
0-54 |
Sentence |
denotes |
2.7.2 Tissue tropism of early viral infection in vivo |
| T99 |
55-141 |
Sentence |
denotes |
A total of 100 1-day-old SPF chickens were assigned to four groups (n = 25 per group). |
| T100 |
142-404 |
Sentence |
denotes |
The birds were inoculated with 200 μL allantoic fluid containing 105 EID50 of the rH120, rIBYZ, or rH120-S1/YZ strains, respectively via eye drop and the intranasal route and 200 μL PBS per chick was administered to the control group (n = 25) via the same route. |
| T101 |
405-590 |
Sentence |
denotes |
The tracheas, lungs, kidneys, and bursa from the 5 inoculated birds per group were harvested at 1, 3, 5, and 7 dpi, weighed, and collected into 1 mL PBS per sample and frozen at −80 °C. |
| T102 |
591-797 |
Sentence |
denotes |
After grinding the samples, the viral RNA was extracted using TRIzol, and the cDNA was obtained by reverse transcription using a PrimeScript RT Master Mix Perfect Real Time Kit (TaKaRa, Otsu, Shiga, Japan). |
| T103 |
798-904 |
Sentence |
denotes |
The viral RNA copies from each of the different samples were detected by real-time PCR as described above. |
| T104 |
905-1021 |
Sentence |
denotes |
All assays were run in triplicate and the copy number for each virus was calculated according to the standard curve. |
