LitCovid-sentences  

PMC:7115396 / 13641-16952 JSONTXT 10 Projects

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Id Subject Object Predicate Lexical cue
T89 0-19 Sentence denotes 2.7 Safety testing
T90 21-83 Sentence denotes 2.7.1 Assessment of pathogenicity in one-day-old SPF chickens
T91 84-205 Sentence denotes The chickens were separately housed in isolators under consistent conditions, and food and water was provided ad libitum.
T92 206-304 Sentence denotes A total of 120 one-day-old SPF chickens were randomly divided into four groups (n = 30 per group).
T93 305-477 Sentence denotes The chickens in the experimental groups were intranasally inoculated with 200 μL allantoic fluid per chick containing 105EID50 of the rH120, rIBYZ, and rH120-S1/YZ strains.
T94 478-563 Sentence denotes The control group (n = 30) was inoculated with 200 μL sterile PBS via the same route.
T95 564-620 Sentence denotes The morbidity and mortality was followed-up for 14 days.
T96 621-792 Sentence denotes All experimental groups were monitored daily for clinical signs related to IB infection for 14 days post-infection (dpi), including coughing, sneezing, and tracheal rales.
T97 793-866 Sentence denotes Dead chickens were examined for gross tracheal, lung, and kidney lesions.
T98 868-922 Sentence denotes 2.7.2 Tissue tropism of early viral infection in vivo
T99 923-1009 Sentence denotes A total of 100 1-day-old SPF chickens were assigned to four groups (n = 25 per group).
T100 1010-1272 Sentence denotes The birds were inoculated with 200 μL allantoic fluid containing 105 EID50 of the rH120, rIBYZ, or rH120-S1/YZ strains, respectively via eye drop and the intranasal route and 200 μL PBS per chick was administered to the control group (n = 25) via the same route.
T101 1273-1458 Sentence denotes The tracheas, lungs, kidneys, and bursa from the 5 inoculated birds per group were harvested at 1, 3, 5, and 7 dpi, weighed, and collected into 1 mL PBS per sample and frozen at −80 °C.
T102 1459-1665 Sentence denotes After grinding the samples, the viral RNA was extracted using TRIzol, and the cDNA was obtained by reverse transcription using a PrimeScript RT Master Mix Perfect Real Time Kit (TaKaRa, Otsu, Shiga, Japan).
T103 1666-1772 Sentence denotes The viral RNA copies from each of the different samples were detected by real-time PCR as described above.
T104 1773-1889 Sentence denotes All assays were run in triplicate and the copy number for each virus was calculated according to the standard curve.
T105 1891-1912 Sentence denotes 2.7.3 Histopathology
T106 1913-2165 Sentence denotes After inoculation with 105 EID50 dose of the rH120, rIBYZ, and rH120-S1/YZ strains, three infected chickens per group were randomly selected to be euthanized by bleeding, and the tissue pathology was examined in the different groups at 3, 5, 7, 10 dpi.
T107 2166-2390 Sentence denotes The tracheas, lungs, kidneys, and bursas of three dead or other randomly-selected chickens from the experimental and control groups (inoculated with PBS) were collected at 5 or 7 dpi and further processed for histopathology.
T108 2391-2512 Sentence denotes The samples were fixed in formalin, embedded in paraffin, cut into 5 μm sections, and stained with hematoxylin and eosin.
T109 2513-2589 Sentence denotes The slides were examined under light microscopy for the presence of lesions.
T110 2591-2612 Sentence denotes 2.7.4 Viral shedding
T111 2613-2827 Sentence denotes To determine the level of viral shedding of the different recombinants, 10 birds per group were inoculated with 200 μL allantoic fluid containing 105 EID50 of the rH120, rIBYZ, or rH120-S1/YZ strains, respectively.
T112 2828-2927 Sentence denotes 200 μL PBS per chick was administered to the control group via eye drop and the intranasal route. .
T113 2928-3049 Sentence denotes Oral and cloacal swabs were collected from each bird into 1 mL PBS at 7, 14, 18, 22, 26, and 30 dpi and stored at −80 °C.
T114 3050-3311 Sentence denotes After freezing and thawing three times and centrifuging at 8,000 × g for 5 min at 4 °C, relative amount of virus present in 200 μL of supernatants of each sample was quantified by RNA extraction, reverse transcription, and real-time PCR as described previously.