| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T81 |
0-34 |
Sentence |
denotes |
2.6 Titration of rIBVs in embryos |
| T82 |
35-278 |
Sentence |
denotes |
Serial 10-fold dilutions from 105 copies to 109 copies per 0.1 mL of virus of the recombinant strain, rH120-S1/YZ, and its parental strains (rH120 and rIBYZ) were inoculated into the allantoic cavity of 10-day-old embryonated SPF chicken eggs. |
| T83 |
279-394 |
Sentence |
denotes |
For each dilution, 0.1 mL of the virus solution was injected into each egg and 10 eggs were used for each dilution. |
| T84 |
395-430 |
Sentence |
denotes |
PBS was used as a negative control. |
| T85 |
431-522 |
Sentence |
denotes |
After a 24 h incubation, the dead embryos were considered nonspecific deaths and discarded. |
| T86 |
523-631 |
Sentence |
denotes |
The embryos were examined for the presence of specific lesions caused by the virus after a 144-h incubation. |
| T87 |
632-763 |
Sentence |
denotes |
Dead and live embryos that displayed IBV infectious signs (e.g., dwarfing, curling, and stunting) were considered positive samples. |
| T88 |
764-867 |
Sentence |
denotes |
The 50% embryo infectious dose (EID50) of these rIBVs was calculated using the Reed-Muench method [37]. |
