| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T319 |
0-103 |
Sentence |
denotes |
HEK-293T cells were transfected with plasmids encoding SARS-CoV-2 S, SARS-CoV S, or MERS-CoV S protein. |
| T320 |
104-254 |
Sentence |
denotes |
Cells were detached with 1 mM EDTA 40 h post transfection, washed twice with 3% FBS in 1×PBS, and incubated with 5 μg/ml soluble hACE2 for 1 h on ice. |
| T321 |
255-516 |
Sentence |
denotes |
After washing three times with 3% FBS in 1×PBS, cells were incubated with polyclonal goat anti-human ACE2 antibody (1:200) (R&D Systems, MN, USA) for 1 h, followed by FITC-conjugated rabbit anti-goat secondary antibody (1:500) (Jackson ImmunoResearch, PA, USA). |
| T322 |
517-575 |
Sentence |
denotes |
After washing, cells were then analyzed by flow cytometry. |
