| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T297 |
0-48 |
Sentence |
denotes |
Flow cytometric analysis of S protein expression |
| T298 |
49-181 |
Sentence |
denotes |
Briefly, 293T cells were transfected with 2 μg of plasmids encoding either SARS-CoV-2 S, SARS-CoV S or MERS-CoV S protein using PEI. |
| T299 |
182-249 |
Sentence |
denotes |
Forty hours later, cells were detached by using PBS with 1 mM EDTA. |
| T300 |
250-515 |
Sentence |
denotes |
After washing, cells were incubated with polyclonal rabbit anti-SARS S1 antibodies T62 (1:200 dilution) (Sinobiological Inc., Beijing, China) for 1 h on ice, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (1:200) (ZSGB-Bio LLC, Beijing, China) for 1 h. |
| T301 |
516-559 |
Sentence |
denotes |
Cells were then analyzed by flow cytometry. |
