LitCovid-sentences  

PMC:7100515 / 20115-22219 JSONTXT 12 Projects

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Id Subject Object Predicate Lexical cue
T148 0-49 Sentence denotes Cell–cell fusion mediated by SARS-CoV-2 S protein
T149 50-241 Sentence denotes Type II membrane serine proteases (TMPRSS)-mediated cleavage can activate the fusion potential of SARS-CoV and MERS-CoV S protein and induce receptor-dependent syncytium formation28,29,38,39.
T150 242-476 Sentence denotes To test whether SARS-CoV-2 S protein cleaved by these cellular proteases could trigger syncytium formation, we overlaid HEK 293T cells expressing SARS-CoV-2 S or control proteins on 293/hACE2 cells in presence or absence of proteases.
T151 477-645 Sentence denotes Expression of TMPRSS 2, 4, 11 A, 11D, and 11E on 293/hACE2 cells enhanced SARS-CoV-2 S protein-mediated cell–cell fusion similarly to SASR-CoV S (Supplementary Fig. 2).
T152 646-684 Sentence denotes Next we evaluated the role of trypsin.
T153 685-847 Sentence denotes Consistent to our previous report23, addition of trypsin triggered syncytium formation on 293/hACE2 cells by SARS-CoV S protein after 4 h incubation (Fig. 4b, c).
T154 848-1010 Sentence denotes Large syncytia were formed at a level similar to SARS-CoV S proteins when SARS-CoV-2 S protein expressing 293T cells were added onto 293/hACE2 cells with trypsin.
T155 1011-1281 Sentence denotes Of note, SARS-CoV-2, but not SARS-CoV, S proteins induced syncytia formation on 293/hACE2 cells even in the absence of trypsin (Fig. 4c), suggesting that SARS-CoV-2 S proteins could be triggered upon the receptor binding without exogenous protease priming or activation.
T156 1282-1437 Sentence denotes During 2013–2016 Ebola virus outbreak, A82V and T544I mutations in glycoprotein (gp) of Ebola virus were linked to increase of virus transmissibility40–42.
T157 1438-1581 Sentence denotes Further study showed that A82V and T544I mutation decreases the thermostability of gp while they increases virus infectivity in cell culture42.
T158 1582-1647 Sentence denotes We then investigated the thermostability of SARS-CoV-2 S protein.
T159 1648-1806 Sentence denotes Compared to SARS-CoV S protein, SARS-CoV-2 S protein was less stable, requiring significant shorter time and lower temperature to be inactivated (Fig. 4d, e).
T160 1807-2035 Sentence denotes The native S protein is metastable, and there is an energy barrier that prevents it from undergoing conformational change before triggering, SARS-CoV-2 S protein might decrease its energy barrier by reducing its thermostability.
T161 2036-2104 Sentence denotes This might contribute to high-transmission efficiency of SARS-CoV-2.