LitCovid-sentences  

PMC:7100515 / 17363-20113 JSONTXT 11 Projects

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Id Subject Object Predicate Lexical cue
T130 0-187 Sentence denotes Protease activation on S protein is an important step for coronavirus entry, and cathepsins in lysosome have been shown to be critical for SARS-CoV and MERS-CoV entry through endocytosis.
T131 188-435 Sentence denotes To investigate whether cathepsins are required for SARS-CoV-2 entry, HEK 293/hACE2 cells were treated with either a broad inhibitor for cathepsin B, H, L, and calpain (E64D), cathepsin L inhibitor (SID 26681509), or cathepsin B inhibitor (CA-074).
T132 436-562 Sentence denotes VSV-G pseudovirions were used as a negative control, since virus entry mediated by VSV-G does not require protease activation.
T133 563-754 Sentence denotes E64D treatment of 293/hACE2 cells reduced entry of SARS-CoV-2 S pseudovirions by 92.5%, indicating that at least one of cathepsins or calpain might be required for SARS-CoV-2 entry (Fig. 4a).
T134 755-1084 Sentence denotes While cathepsin B inhibitor treatment did not show any marked effect on virus entry, cathepsin L inhibition treatment decreased entry of SARS-CoV-2 S pseudovirions into 293/hACE2 by over 76% (Fig. 4a), suggesting that cathepsin L should be essential for priming of SARS-CoV-2 S protein in lysosome for entry into 293/hACE2 cells.
T135 1085-1152 Sentence denotes Fig. 4 Activation of SARS-CoV-2 S protein by cathepsin and trypsin.
T136 1153-1245 Sentence denotes a Effects of cathepsin inhibitors on entry of SARS-CoV-2 S pseudovirions on 293/hACE2 cells.
T137 1246-1480 Sentence denotes HEK 293/hACE2 cells were pretreated with broad-spectrum cathepsin inhibitor E64D, cathepsin L-specific inhibitor (SID 26681509), or cathepsin B-specific inhibitor (CA-074) and then transduced with SARS-CoV-2 S and VSV-G pseudovirions.
T138 1481-1544 Sentence denotes Pseudoviral transduction was measured at 40 h post inoculation.
T139 1545-1616 Sentence denotes Experiments were done in triplicates and repeated at least three times.
T140 1617-1645 Sentence denotes One representative is shown.
T141 1646-1748 Sentence denotes Error bars indicate SEM of technical triplicates. b Cell–cell fusion mediated by SARS-CoV-2 S protein.
T142 1749-1945 Sentence denotes HEK 293T cells were transiently expressing eGFP and either SARS-CoV-2 or SARS-CoV S protein were detached with either trypsin or EDTA, and co-cultured with 293/hACE2 or 293 cells for 4 h at 37 °C.
T143 1946-2242 Sentence denotes The scale bar indicates 250 µm. c Quantitative analysis of syncytia in panel b. d, e Thermostability analysis of SARS-CoV-2 S protein. d SARS-CoV and SARS-CoV-2 S pseudovirons were incubated at 37 °C for the specified times (0 to 4 h) in the absence of serum, and then assayed on 293/hACE2 cells.
T144 2243-2545 Sentence denotes The results from infection at 0 h were set as 100%, and the experiments were repeated four times, and means with standard deviations are shown. e SARS-CoV and SARS-CoV-2 S pseudovirions without serum were incubated at the indicated temperature (37 to 51 °C) for 2 h and then assayed on 293/hACE2 cells.
T145 2546-2614 Sentence denotes The results are reported as the percentage of transduction at 37 °C.
T146 2615-2702 Sentence denotes The experiments were repeated four times, and means with standard deviations are shown.
T147 2703-2750 Sentence denotes Source data are provided as a Source Data file.