| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T50 |
0-41 |
Sentence |
denotes |
SARS-CoV-2/Finland/1/2020 virus isolation |
| T51 |
42-254 |
Sentence |
denotes |
The SARS-CoV-2 virus SARS-CoV-2/Finland/1/2020 was isolated in a biosafety level 3 (BSL-3) laboratory in Vero E6 cells from the Day 4 nasopharyngeal swab (NPS) and nasopharyngeal aspirate (NPA) specimens (Table). |
| T52 |
255-541 |
Sentence |
denotes |
The samples were inoculated into the cells for 1 h at 37 °C and 5% CO2 and fresh culture medium (Eagle's minimum essential medium (EMEM) supplemented with 2% fetal bovine serum (FBS), 0.6 μg/mL penicillin, 60 μg/mL streptomycin, 2 mM L-glutamine, 20 mM HEPES) were added for incubation. |
| T53 |
542-695 |
Sentence |
denotes |
On the 4th day of incubation, half of the cultures were blind-passaged onto fresh Vero E6 cells and the rest of original passages were incubated further. |
| T54 |
696-777 |
Sentence |
denotes |
After 4 days incubation a clear CPE was detected in the NPA-originated passage 2. |
| T55 |
778-924 |
Sentence |
denotes |
The propagation of stock virus was done by passaging a low virus dose once again in Vero E6 cells, and virus culture was harvested on the 3rd day. |
| T56 |
925-968 |
Sentence |
denotes |
Virus concentration was followed by RT-PCR. |
| T57 |
969-1175 |
Sentence |
denotes |
The Ct value for virus passage 1 on the 6th day of incubation was 17.65 and for passage 2 on the 2nd day, before any CPE was 20.63, whereas those of the NPS specimen remained at Ct values between 35 and 36. |
