| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T302 |
0-24 |
Sentence |
denotes |
Immunofluorescence Assay |
| T303 |
25-153 |
Sentence |
denotes |
Vero E6 cells were seeded in eight-well chamber slides and incubated overnight prior to infection with MERS-CoV at M.O.I. = 0.1. |
| T304 |
154-293 |
Sentence |
denotes |
The cells were fixed with 4% (v/v) paraformaldehyde for 20 min at 4 °C, followed by permeabilization in 0.1% (v/v) Triton X-100 for 10 min. |
| T305 |
294-365 |
Sentence |
denotes |
Then, 7.5% (v/v) BSA was used as a blocking buffer for 30 min at 37 °C. |
| T306 |
366-482 |
Sentence |
denotes |
Anti-MERS-CoV N primary antibody (1:500 dilution; Sino Biological Inc., Beijing, China) was used to stain the virus. |
| T307 |
483-693 |
Sentence |
denotes |
The cells were incubated overnight, washed thrice with PBS, and incubated with Alex Fluor 568 anti-rabbit secondary antibody (1:1000 dilution; Thermo Fisher Scientific, Waltham, MA) for 1 h at room temperature. |
| T308 |
694-761 |
Sentence |
denotes |
4′,6-Diamidino-2-phenylindole (DAPI) was added during the PBS wash. |
| T309 |
762-878 |
Sentence |
denotes |
MERS nucleocapsid expression was examined under a confocal microscope (LSM-700; Carl Zeiss AG, Oberkochen, Germany). |
