| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T22 |
0-94 |
Sentence |
denotes |
Here, we report the crystal structure of MERS-CoV N-NTD in a non-native dimeric configuration. |
| T23 |
95-199 |
Sentence |
denotes |
We used the non-native dimer interface as the target in virtual screening for an orthosteric stabilizer. |
| T24 |
200-383 |
Sentence |
denotes |
To this end, we considered the binding scores and hydrophobic complementarity of the acquired poses, and further selected the potential leads P1–P3 from Acros and ZINC drug databases. |
| T25 |
384-486 |
Sentence |
denotes |
Of these, only 5-benzyloxygramine (P3) had both antiviral and stabilizing activities on the N protein. |
| T26 |
487-646 |
Sentence |
denotes |
Small-angle X-ray scattering (SAXS) and cell-based assays showed that P3 induces abnormal full-length N protein oligomerization in vitro and at the cell level. |
| T27 |
647-770 |
Sentence |
denotes |
We also described the structure of MERS-CoV N-NTD complexed with 5-benzyloxygramine and revealed its stabilizing mechanism. |
| T28 |
771-915 |
Sentence |
denotes |
Our findings provide insight into the development of a new therapeutic approach based on stabilizing a non-native protein interaction interface. |
| T29 |
916-1011 |
Sentence |
denotes |
It may lead to the discovery and development of new treatments for various infectious diseases. |
