LitCovid-sentences  

PMC:7091888 / 4966-6821 JSONTXT 8 Projects

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Id Subject Object Predicate Lexical cue
T18 0-151 Sentence denotes By alignment of the RBD sequences of SARS-CoV and SARS-CoV-2, we identified the region of SARS-CoV-2 RBD at residues 331 to 524 of S protein (Fig. 1a).
T19 152-453 Sentence denotes We then constructed a recombinant RBD protein containing codon-optimized RBD sequences with a C-terminal Fc of human IgG1 (hFc) using pFUSE-hIgG1-Fc2 expression vector, expressed the protein in mammalian cell 293T, and purified it from cell culture supernatant using protein A affinity chromatography.
T20 454-581 Sentence denotes Similar to SARS-CoV and MERS-CoV RBD protein controls, SARS-CoV-2 RBD protein had high expression with strong purity (Fig. 1b).
T21 582-927 Sentence denotes Notably, only SARS-CoV-2 and SARS-CoV RBDs were recognized by SARS-CoV RBD-specific, but not MERS-CoV RBD-specific, polyclonal antibodies (Fig. 1c), whereas only MERS-CoV RBD was recognized by MERS-CoV RBD-immunized polyclonal antibodies (Fig. 1d), suggesting the cross-reactivity of SARS-CoV RBD-specific antibodies with SARS-CoV-2 RBD protein.
T22 928-1066 Sentence denotes Fig. 1 Characterization of SARS-CoV-2 RBD. a Multiple sequence alignment of RBDs of SARS-CoV-2, SARS-CoV, and MERS-CoV spike (S) proteins.
T23 1067-1177 Sentence denotes GenBank accession numbers are QHR63250.1 (SARS-CoV-2 S), AY278488.2 (SARS-CoV S), and AFS88936.1 (MERS-CoV S).
T24 1178-1354 Sentence denotes Variable amino acid residues between SARS-CoV-2 and SARS-CoV are highlighted in cyan, and conserved residues among SARS-CoV-2, SARS-CoV, and MERS-CoV are highlighted in yellow.
T25 1355-1492 Sentence denotes Asterisks represent fully conserved residues, colons represent highly conserved residues, and periods represent lowly conserved residues.
T26 1493-1541 Sentence denotes The alignment was performed using Clustal Omega.
T27 1542-1606 Sentence denotes SDS-PAGE (b) and Western blot (c, d) analysis of SARS-CoV-2 RBD.
T28 1607-1674 Sentence denotes The protein molecular weight marker (kDa) is indicated on the left.
T29 1675-1728 Sentence denotes SARS-CoV and MERS-CoV RBDs were included as controls.
T30 1729-1855 Sentence denotes Antisera (1:3,000 dilution) from mice immunized with SARS-CoV RBD (c) and MERS-CoV RBD (d) were used for Western blot analysis