| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T168 |
0-304 |
Sentence |
denotes |
SARS-CoV-2 pseudovirus was generated, as previously described, with some modifications.25,27,29 Briefly, 293T cells were cotransfected with a plasmid encoding Env-defective, luciferase-expressing HIV-1 genome (pNL4-3.luc.RE) and a plasmid encoding SARS-CoV-2 S protein using the calcium phosphate method. |
| T169 |
305-367 |
Sentence |
denotes |
SARS-CoV and MERS-CoV pseudoviruses were packaged as controls. |
| T170 |
368-576 |
Sentence |
denotes |
The transfected medium was changed into fresh Dulbecco’s Modified Eagle’s Medium (DMEM) 8 h later, and pseudovirus-containing supernatants were collected 72 h later for single-cycle infection in target cells. |
| T171 |
577-943 |
Sentence |
denotes |
Pseudovirus neutralization assay was then performed by incubation of SARS-CoV-2, SARS-CoV, or MERS-CoV pseudovirus with serially diluted SARS-CoV RBD- or MERS-CoV RBD-immunized mouse sera for 1 h at 37 °C, followed by addition of the mixture into hACE2/293T (for SARS-CoV-2 pseudovirus and SARS-CoV pseudovirus) or hDPP4/293T (for MERS-CoV pseudovirus) target cells. |
| T172 |
944-1059 |
Sentence |
denotes |
Fresh medium was added 24 h later, and the cells were lysed 72 h later in cell lysis buffer (Promega, Madison, WI). |
| T173 |
1060-1230 |
Sentence |
denotes |
The lysed cell supernatants were incubated with luciferase substrate (Promega) and detected for relative luciferase activity using the Infinite 200 PRO Luminator (Tecan). |
| T174 |
1231-1372 |
Sentence |
denotes |
The 50% MERS pseudovirus neutralizing antibody titer (NT50) was calculated using the CalcuSyn computer program, as previously described.29,35 |
