| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T159 |
0-5 |
Sentence |
denotes |
ELISA |
| T160 |
6-202 |
Sentence |
denotes |
ELISA was performed to detect the binding of SARS-CoV-2 RBD protein to sACE2 receptor, as previously described.27,32,34 SARS-CoV and MERS-CoV RBDs, as well as sDPP4 protein, were used as controls. |
| T161 |
203-370 |
Sentence |
denotes |
Briefly, ELISA plates were precoated with SARS-CoV-2, SARS-CoV, or MERS-CoV RBD (1 μg/ml) overnight at 4 °C and blocked with 2% fat-free milk in PBST for 2 h at 37 °C. |
| T162 |
371-468 |
Sentence |
denotes |
Serially diluted sACE2, or sDPP4, protein was added to the plates and incubated for 2 h at 37 °C. |
| T163 |
469-725 |
Sentence |
denotes |
After four washes, the bound protein was detected using hACE2- or hDPP4-specific goat antibody (0.5 μg/ml, R&D system) for 2 h at 37 °C, followed by incubation with HRP-conjugated anti-goat IgG antibody (1:5,000, Thermo Fisher Scientific) for 1 h at 37 °C. |
| T164 |
726-866 |
Sentence |
denotes |
The reaction was visualized by addition of substrate 3,3’,5,5’-Tetramethylbenzidine (TMB) (Sigma, St. Louis, MO) and stopped by H2SO4 (1 N). |
| T165 |
867-959 |
Sentence |
denotes |
The absorbance at 450 nm (A450) was measured by an ELISA plate reader (Tecan, San Jose, CA). |
| T166 |
960-1362 |
Sentence |
denotes |
The cross-reactivity of SARS-CoV-2 RBD protein to SARS-CoV RBD-specific antibody was performed by coating ELISA plates with SARS-CoV-2 RBD (1 μg/ml), as well as SARS-CoV RBD or MERS-CoV RBD (as controls, 1 μg/ml), followed by sequential incubation with serially diluted SARS-CoV RBD- or MERS-CoV RBD-immunized mouse sera and HRP-conjugated anti-mouse IgG (1:5,000; Thermo Fisher Scientific) antibodies. |
