Id |
Subject |
Object |
Predicate |
Lexical cue |
T119 |
0-275 |
Sentence |
denotes |
Since SARS-CoV-2 is more phylogenetically related to SARS-CoV than MERS-CoV,22 we further detected the cross-reactivity of SARS-CoV RBD-specific antibodies with SARS-CoV-2 RBD and cross-neutralizing activity of SARS-CoV RBD-specific antibodies against pseudotyped SARS-CoV-2. |
T120 |
276-393 |
Sentence |
denotes |
First, we performed an ELISA to detect the cross-reactivity of SARS-CoV RBD-immunized mouse sera with SARS-CoV-2 RBD. |
T121 |
394-581 |
Sentence |
denotes |
The results showed that SARS-CoV-2 RBD reacted strongly with anti-SARS-CoV RBD IgG with antibody titer of 1:2.4 × 104 (Fig. 4d), but it did not react with anti-MERS-CoV RBD IgG (Fig. 4e). |
T122 |
582-668 |
Sentence |
denotes |
As expected, SARS-CoV RBD reacted strongly with anti-SARS-CoV RBD IgG (antibody titer: |
T123 |
669-738 |
Sentence |
denotes |
1:1.4 × 105) (Fig. 4d), but not with anti-MERS-CoV RBD IgG (Fig. 4e). |
T124 |
739-867 |
Sentence |
denotes |
MERS-CoV RBD did not react with anti-SARS-CoV RBD IgG (Fig. 4d), but instead reacted with anti-MERS-CoV RBD IgG (antibody titer: |
T125 |
868-891 |
Sentence |
denotes |
1:1.3 × 105) (Fig. 4e). |
T126 |
892-1068 |
Sentence |
denotes |
Second, we performed a pseudovirus neutralization assay to detect the cross-neutralizing activity of SARS-CoV RBD-immunized mouse sera against SARS-CoV-2 pseudovirus infection. |
T127 |
1069-1350 |
Sentence |
denotes |
Results revealed that SARS-CoV RBD-specific antisera could neutralize SARS-CoV-2 pseudovirus infection with a neutralizing antibody titer of 1:323, while these antisera could neutralize SARS-CoV pseudovirus infection with higher neutralizing antibody titer (1:1.2 × 104) (Fig. 4f). |
T128 |
1351-1619 |
Sentence |
denotes |
MERS-CoV RBD-inducing mouse sera only neutralized MERS-CoV pseudovirus infection in hDPP4-expressing cells with a neutralizing antibody titer of 1:4 × 104 (Fig. 4g), but failed to neutralize infection by either SARS-CoV-2 pseudovirus or SARS-CoV pseudovirus (Fig. 4f). |
T129 |
1620-1767 |
Sentence |
denotes |
These data suggest that SARS-CoV RBD-specific antibodies can cross-react with SARS-CoV-2 RBD and cross-neutralize SARS-CoV-2 pseudovirus infection. |