| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T84 |
0-84 |
Sentence |
denotes |
Fig. 3 Comparison of SARS-CoV-2 RBD protein binding to human and bat ACE2 receptors. |
| T85 |
85-227 |
Sentence |
denotes |
Flow cytometry analysis of SARS-CoV-2 RBD binding to hACE2 and bat ACE2 (bACE2) receptors in 293T cells transiently expressing hACE2 or bACE2. |
| T86 |
228-377 |
Sentence |
denotes |
293T cells were transiently transfected with hACE2 or bACE2 plasmid and incubated with SARS-CoV-2 RBD protein at various concentrations for analysis. |
| T87 |
378-439 |
Sentence |
denotes |
SARS-CoV RBD and MERS-CoV RBD proteins were used as controls. |
| T88 |
440-587 |
Sentence |
denotes |
Representative images of SARS-CoV-2 RBD protein (2.5 μg/ml) binding to bACE2/293T (a, black line), or hACE2/293T (c, black line), cells were shown. |
| T89 |
588-722 |
Sentence |
denotes |
Binding of SARS-CoV RBD protein (2.5 μg/ml) to bACE2/293T (b, red line), or hACE2/293T (d, red line), cells were used as a comparison. |
| T90 |
723-955 |
Sentence |
denotes |
MERS-CoV RBD protein (green line) and mock-incubated (gray shading) cells (a–d) were included as controls. e, f Dose-dependent binding of SARS-CoV-2 RBD protein to bACE2/293T (e), or hACE2/293T (f), cells by flow cytometry analysis. |
| T91 |
956-1147 |
Sentence |
denotes |
Significant differences between binding of SARS-CoV-2 RBD (black) and SARS-CoV RBD (red) to cell-associated bACE2 receptor (e), or hACE2 receptor (f) were identified based on the EC50 values. |
| T92 |
1148-1196 |
Sentence |
denotes |
The data are presented as mean ± s.e.m. (n = 4). |
| T93 |
1197-1256 |
Sentence |
denotes |
Experiments were repeated twice and yielded similar results |
